Where does monochlorobimane bins onto GSH?
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Monochlorobimane (MCB) binds onto glutathione (GSH) primarily through interactions with glutathione S-transferase enzymes . Studies have shown that MCB has a high affinity for rat neutral transferases, with a Km of 2.6 microM, indicating excellent substrate reactivity. In contrast, human transferases, especially the pi isoform prevalent in human tumor cell lines, exhibit reduced affinity for MCB, with a Km of 264 microM, resulting in lower labeling efficiency at lower concentrations (<50 microM) in human cell lines. Additionally, the nuclear/cytoplasmic distribution of MCB-GSH conjugates in rat hepatocytes suggests that MCB diffuses into the nucleus and accumulates there, limiting its value in estimating GSH concentrations in the nucleus and cytoplasm.
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| Papers (5) | Insight |
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Open access•Journal Article | Monochlorobimane binds onto glutathione (GSH) primarily through interactions with different glutathione S-transferase isozymes, showing varying affinities for human and rodent enzymes. |
01 Feb 2021 2 Citations | Monochlorobimane binds onto GSH via S-glutathionylation in the developing zebrafish embryo, allowing visualization of GSH changes at a suborganism level. |
| Monochlorobimane (MCB) binds onto glutathione (GSH) in living cells, allowing for the assessment of cell redox status through relative GSH content and responsiveness to various factors. | |
54 Citations | Monochlorobimane binds onto GSH in the nucleus and cytosol of isolated hepatocytes, diffusing and accumulating primarily in the nucleus, affecting accurate GSH concentration estimations. |
37 Citations | Monochlorobimane binds onto glutathione S-transferase, causing challenges in labeling GSH in certain human tumor cell lines, requiring higher dye concentrations and longer staining times for adequate staining. |
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