Yield of cell disruption of microalgae using bead mill as a cell lysis method? in percentage?
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Bead milling as a cell disruption method for microalgae has shown varying efficiencies in different studies. In the case of Coelastrella sp., bead milling was found to have a rupture efficiency above 90% after treatment, making it one of the most efficient methods for cell disruption . On the other hand, a study on Nannochloropsis oceanica, Nannochloropsis gaditana, and Tetraselmis suecica found that bead milling was effective in extracting 12.6% dry weight of lipids from T. suecica, indicating a lower efficiency compared to other methods for this specific microalgae strain . Additionally, a simulation study on microalgae lipid production highlighted that scenarios involving bead milling had higher energy consumption and operating costs compared to other cell disruption methods like high-pressure homogenization .
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| Papers (5) | Insight |
|---|---|
| Bead milling as a cell disruption method for microalgae can yield up to 75% lipid extraction efficiency when combined with chemical extraction, as reported in the study. | |
11 Citations | Explosive decompression method yields higher cell disruption efficiency compared to bead milling, with over 2-fold increase in biomass release efficiency, but exact percentage values are not provided in the abstract. |
17 Feb 2021 | Bead milling achieved a 12.6% dry weight lipid extraction from Tetraselmis suecica, as the most effective method for this strain in the study. |
| Bead milling as a cell disruption method for Coelastrella sp. resulted in a cell rupture efficiency of around 90%, facilitating the release of intracellular organic compounds efficiently. | |
| Not addressed in the paper. |
Related Questions
Disadvantages of using bead mill to cell disruption of microalgae?5 answersDisadvantages of using bead milling for cell disruption of microalgae include high energy consumption and operating costs compared to other methods like high-pressure homogenization (HPH). Bead milling can also lead to underestimation of cell disruption yield when manual counting methods are used, potentially affecting the accuracy of results. Additionally, bead milling may not be as effective in disrupting certain strains of microalgae, such as Tetraselmis suecica, as other methods like microwaves or ultrasound. Despite being efficient in releasing intracellular organic matter, bead milling can be less effective in disrupting cells compared to methods like sonication, which have shown higher disruption efficiencies in various studies.
Disadvantages of using ultrasonication to cell disruption of microalgaeC. vulgaris?5 answersDisadvantages of using ultrasonication for cell disruption of microalgae C. vulgaris include varying susceptibility to rupture based on growth medium, limited efficiency compared to other methods like pressure-assisted ozonation, and differing disruption rates based on the biological and physical properties of the microalgae species. Ultrasonication may not consistently achieve high disruption efficiencies, with studies showing that other methods like pressure-assisted ozonation can outperform it in terms of cell rupture percentages. Additionally, the effectiveness of ultrasonication can be influenced by factors such as the composition of the growth medium and the specific characteristics of the microalgae species being treated. These factors can impact the overall efficiency and reliability of ultrasonication as a cell disruption method for microalgae C. vulgaris.
Bead mill disadvantages for microalgae cell lysis?5 answersBead mills, commonly used for microalgae cell disruption, have some disadvantages. One major drawback is the variability in disruption efficiency based on the physiological state of the microalgae cells. Research on Tetraselmis chui and Chlorella cells showed that differences in cell morphology and physiological state significantly impacted cell disruption efficiency and protein recovery yields during bead milling. Additionally, traditional bead milling methods can be time-consuming and demand high energy input, leading to inefficient disruption and lower biomass release compared to newer methods like CO2-based explosive decompression. Furthermore, while bead mills are effective in disrupting cells, they can be harsh on sensitive products due to the high shear rates involved, potentially affecting the recovery of delicate compounds from microalgae.
Yield of efficiency of bead milling for cell disruption of c. vulgaris?4 answersBead milling has been proven to be an efficient method for cell disruption of C. vulgaris, leading to high yields of bioactive compounds. Studies have shown that bead milling can achieve significant lipid extraction from C. vulgaris, with extraction yields ranging from 13.9% to 17.6% using different solvent combinations. Additionally, bead milling has been highlighted as a method that can handle a large volume of cells, making it economical and productive, especially when operated in a continuous mode. The release kinetics of pDNA in a bead mill have also been studied, demonstrating its efficiency for the release of important biomolecules from C. vulgaris cells. Overall, bead milling stands out as a promising and effective technique for cell disruption of C. vulgaris, offering high efficiency and optimal yields of bioactive compounds.
Methods/processes that have extracted proteins from C.vulgaris microalgae by bead milling?5 answersBead milling has been identified as an effective method for extracting proteins from C. vulgaris microalgae. Studies have shown that bead milling, when applied to C. vulgaris, resulted in efficient cell disruption and protein release, with varying outcomes based on the specific conditions and characteristics of the microalgae. Research has highlighted that bead milling can lead to significant protein solubilization, with protein recovery yields ranging from 11% to 32% depending on the physiological state of the microalgae. Additionally, the combination of bead milling with other disruptive processes, such as pulsed electric fields (PEF) and high-pressure homogenization (HPH), has been explored to enhance the selectivity of extraction and improve the yields of carbohydrates and lipids, further showcasing the versatility and efficiency of bead milling in protein extraction from C. vulgaris microalgae.
How can a standard curve be used to determine the concentration of a microalgae sample?5 answersA standard curve can be used to determine the concentration of a microalgae sample by correlating the optical density with the number of cells. Traditionally, the detection process for microalgae concentration has been cumbersome and laborious. However, recent studies propose new methodologies for efficient concentration assessment. One such method involves using image analysis to measure the concentration of microalgae species. Another method involves using an automatic calorimetry device that provides a stable test environment and increases the accuracy of the microalgae growth curve. Additionally, a method has been developed that uses a calibration curve to determine a concentration region for a measurement of a response value. These different approaches offer alternatives to the traditional methods and provide more efficient and accurate ways to determine the concentration of microalgae samples.