Showing papers on "Acetylthiocholine published in 2004"
TL;DR: The results indicate that AChE is the enzyme present in brain, whereas in both liver and muscle, the presence of atypical ChEs are suggested, indicating that characterization of ChE is necessary prior to use in monitoring programs.
71 citations
TL;DR: The results predict that the amino acid sequence involved in AChE sensitivity differs in these fishes, and that consequently some fish species may be resistant to the toxicity of methyl-paraoxon.
52 citations
TL;DR: The power of chosen carbamates and hydrazinium derivatives (carbazates) to inhibit the hydrolysis of acetylthiocholine by butyrylcholinesterase or acetylcholineterase was tested and compared with commercially used drugs for the Alzheimer’s disease treatment.
Abstract: The power of chosen carbamates and hydrazinium derivatives (carbazates) to inhibit the hydrolysis of acetylthiocholine by butyrylcholinesterase or acetylcholinesterase was tested. The determined pI 5 0 values (= negative logarithm of the molar concentration inhibiting the enzyme activity by 50%) of the tested substances were compared with pI 5 0 values of the commercially used drugs for the Alzheimer's disease treatment - rivastigmine and galanthamine.
32 citations
Journal Article•
TL;DR: A procedure is suggested for measuring acetylcholinesterase and butyrylcholiersterase activities in human whole blood using acetylthiocholine as a substrate and ethopropazine as a selective inhibitor of butyriesterase.
Abstract: A procedure is suggested for measuring acetylcholinesterase and butyrylcholinesterase activities in human whole blood using acetylthiocholine as a substrate and ethopropazine as a selective inhibitor of butyrylcholinesterase. The procedure is suitable for screening cholinesterase activities in routine and/or field tests.
19 citations
01 Jan 2004
TL;DR: In this article, the mechanism of the colorimetric reaction of thiocholine with Ellman's reagent (5,5'-dithiobis-2-nitrobenzoic acid, RSSR) has been studied.
Abstract: The mechanism of the colorimetric reaction of thiocholine with Ellman 's reagent (5,5'-dithiobis-2-nitrobenzoic acid, RSSR) has been studied. It was found that its mechanism is more complex than acknowledged so far and conforms to two consecutive competitive steps (2) and (3) with the condition for the rate constants k 2 >> k 1 . The applicability of this reaction to the very frequent on-line monitoring of the enzymatic hydrolysis of acetylthiocholine fortunately has not changed. The determined value of absorption coefficient of the measured yellow substance atpH = 8 (5-mercapto-2-nitrobenzoic acid, RSH) is e(412) = 14950 ′ 840 M - 1 cm - 1 .
3 citations
20 Sep 2004