Showing papers on "Brilliant green published in 1985"
TL;DR: It was concluded that addition of MG to BPW may improve the possibility of isolating salmonellas from heavily contaminated materials by limiting the competitive growth of Gram-positive bacteria and the subsequent lowering of the pH of the broth.
Abstract: Large numbers of competitive bacteria may hinder the isolation of salmonellas from food and environmental samples when a pre-enrichment method is used. The addition of 0.1 g/l of malachite green (MG) to buffered peptone water (BPW) inhibited the multiplication of Gram-positive bacteria. Brilliant green had a similar effect but only when the normal recommended concentration of 0.02 g/l was raised to 0.05 g/l. Pure strains of salmonellas were inhibited by MG in BPW, but addition of non fat dried milk (NFDM) (5 g/l or more) counteracted this effect. MG did not affect the recovery of salmonellas injured by heat, freezing, low water activity or acidity in BPW with NFDM. It was concluded that addition of MG to BPW may improve the possibility of isolating salmonellas from heavily contaminated materials by limiting the competitive growth of Gram-positive bacteria and the subsequent lowering of the pH of the broth.
39 citations
TL;DR: The method is simple, inexpensive and technically straightforward and for most isolates gives an identification in 24 h and for 594 of 623 routinely isolated yeasts, the disk-diffusion and the commercial API 20C auxanogram tests gave the same identification.
Abstract: Summary
A disk-diffusion method for identification of yeasts was developed that depended on their different but distinct susceptibilities to the following chemicals: janus green, ethidium bromide, 2, 3, 5-triphenyltetrazolium chloride, brilliant green, cycloheximide and rhodamine 6G. For 594 of 623 routinely isolated yeasts, the disk-diffusion and the commercial API 20C auxanogram tests gave the same identification, an agreement of 95.3%. Only 8 of 1052 isolates from clinical specimens were not identified by the disk-diffusion method. The method is simple, inexpensive and technically straightforward and for most isolates gives an identification in 24 h.
18 citations
Patent•
04 May 1985
TL;DR: In this article, the authors proposed a method to obtain a measuring material discoloring reversibly and clearly according to humidity by preparing a methanol solution containing a specified color developing indicator, a specified inorganic water absorbent and a weak acid such as oxalic acid, and then drying said member in a specified temp range.
Abstract: PURPOSE:To obtain a measuring material discoloring reversibly and clearly according to humidity by preparing a methanol solution containing a specified color developing indicator, a specified inorganic water absorbent and a weak acid such as oxalic acid, dipping a liquid absorbing member into the solution, and then drying said member in a specified temp range CONSTITUTION:The methanol soln containing a mixture or either of brilliant green or crystal violet as the color developing indicator, a mixture or either of ZnBr or magnesium perchlorate as water absorbent, and weak acid such as oxalic acid as acid is prepared The liquid absorbing member such as filter paper is dipped in the solution for 2-3sec, then dried at 70-80 degC to obtain a humidity measuring material The color of said material varies reversibly orange yellow soil color light green green black green purple according to the humidity The humidity in room, hot house, concrete surface, etc can be judged simply by using these colors
11 citations
01 Jan 1985
TL;DR: A comparison of the contrast between Dye-Assis ted and Ion-pair Chromatography and its Applications in Dye Chemistry and Solvent Effects on the Spectrum of Bri l l iant Green 33 shows that the former is more accurate than the latter.
Abstract: X 1 . INTRODUCTION 1 1.1 Histor ical Development 1 1.2 Contrast Between Dye-Assis ted and Ion-pair Chromatography 4 1 .3 Dye Chemistry 7 1 .4 Chromatography Theory 13 2. STATEMENT OF THE PROBLEM 17 3 . EXPERIMENTAL 18 3.1 Chemicals 18 3.2 Apparatus 18 3.3 Experimental Procedures 19 4. DYE CHEMISTRY 2 2 4 .1 Dye Aggregat ion 2 2 4 .2 Solvent Effects on the Spectrum of Bri l l iant Green 33
1 citations