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Showing papers on "Chlorococcum published in 2014"


Journal ArticleDOI
TL;DR: Dairy effluent (DE) was evaluated for cultivation of the oleaginous micro alga Chlorococcum sp.

95 citations


Journal ArticleDOI
TL;DR: A second-order polynomial model was developed and prediction was close to validation experimental results, indicating that the model could be used to guide and optimize the attached culture of Chlorococcum sp.
Abstract: The objective of this study was to understand and optimize the formation of microalgae biofilms in specific culture conditions. Firstly, the adhesion of six freshwater algae species was compared. Chlorococcum sp. was selected because of the high adhesion biomass productivity (ABP) and adhesion rate achieved. Secondly, the adhesion of Chlorococcum sp. was compared with nine commonly used supporting materials, and glass fiber-reinforced plastic proved to be the optimal substrata. Thirdly, based on response surface methodology experiments, a second-order polynomial model was developed to examine the effect of culture period, initial total nitrogen concentration (ITNC) in manure wastewater, pH and culture volume of the growth chamber on the adhesion of Chlorococcum sp. using glass fiber-reinforced plastic. The experimental and modeling results showed that ITNC, pH and culture volume as well as the interactions between culture period and ITNC, culture period and culture volume were significant on ABP. Optimum culture conditions were predicted at a culture period of 11 days, ITNC of 70 mg L−1, pH of 8 and culture volume of 340 mL, under which the predicted maximum ABP was 4.26 g m−2 day−1. The prediction was close to validation experimental results, indicating that the model could be used to guide and optimize the attached culture of Chlorococcum sp. using glass fiber-reinforced plastic.

58 citations


Journal ArticleDOI
TL;DR: In this article, three strains of green microalgae, Chlorococcum sp.C53, Chlorella sp.E53, and Chlora sp.ED53, were studied for their antioxidant activities.
Abstract: Three strains of green microalgae, Chlorococcum sp.C53, Chlorella sp. E53, and Chlorella sp.ED53 were studied for their antioxidant activities. Crude extracts of these microalgae in hot water and in ethanol were examined for their total phenolic contents and for their antioxidant capacities. In order to determine their phenolic contents, the Folin–Ciocalteu method was used. As for the determination of their antioxidant capacities, four different assays were used: (1) total antioxidant capacity determination; (2) DPPH radical scavenging assay; (3) ferrous ion chelating ability assay; and (4) inhibition of lipid peroxidation (using thiobarbituric acid reactive substance). For all the strains we have studied, their ethanolic extract showed more antioxidant activities than their hot water extract. Categorically, the ethanolic extract of Chlorella sp.E53 exhibited both the highest total phenolic content of 35.5 ± 0.14 mg gallic acid equivalent (GAE) g−1 dry weight and the highest DPPH radical scavenging of 68.18 ± 0.38 % at 1.4 mg mL−1 (IC50 0.81 mg mL−1), whereas Chlorella sp.ED53 showed both the highest ferrous ion chelation activity of 42.78 ± 1.48 % at 1 mg mL−1 (IC50 1.23 mg mL−1) and the highest inhibition of lipid peroxidation of 87.96 ± 0.59 % at 4 mg mL−1. This high level of inhibition is comparable to 94.42 ± 1.39 % of butylated hydroxytoluene, a commercial synthetic antioxidant, at the same concentration.

55 citations


Journal ArticleDOI
TL;DR: In this article, the flocculation efficacy of different inorganic and organic amendments was evaluated on various microalgae genera and showed that widely available cheaper biopolymers such as rice starch, maize, and potato starch can be promising flocculants due to their better harvesting efficiency and low price.
Abstract: Cost-efficient harvesting of microalgae is a major challenge due to their small size and often low concentration in the culture medium. The flocculation efficacy of different inorganic and organic amendments was evaluated on various microalgae genera—one strain each belonging to Chlamydomonas, Chlorococcum, two of Botryococcus, and of Chlorella. An improvised medium comprising of commercial grade urea, single super phosphate, and muriate of potash was used to grow the microalgae for flocculation experiments. High pH induced increased flocculation efficiency (72–76 %) in selected microalgal strains. Ferric chloride was found to be the most efficient for most of the microalgal strains, while maize starch and rice starch proved superior for Chlorella sp. MCC6 and Botryococcus sp. MCC32. Although the highest flocculation efficiency was obtained with inorganic flocculant, i.e., ferric chloride (87.3 %) with Botryococcus MCC31, this was comparable with rice starch (86.8 %) for Botryococcus MCC32. This study showed that widely available cheaper biopolymers such as rice starch, maize, and potato starch can be promising flocculants due to their better harvesting efficiency (>80 %) and low price, thereby contributing to economical production of biodiesel from algae.

50 citations


Journal ArticleDOI
TL;DR: In this article, the reactions of a strain of marine alga Chlorococcum sp. and of a polystrain containing a mixture of marine algae with CO/H2O have been studied.
Abstract: The reactions of a strain of marine alga Chlorococcum sp. and of a polystrain containing a mixture of marine algae with CO/H2O have been studied. The yields and chemical composition of the products...

15 citations


Journal Article
TL;DR: Air-dry biomass of microalgae ranged from1.29 to 2.79 g/l of environment, and lipid content of effective strains of Chlorococcum sp.29 and Scenedesmus sp.37 was 41.8 - 58.0% of the total dry biomass ofmicroalgae.
Abstract: From water samples of the Syr-Darya andAmu-Darya are isolated 15 local microalgae belonging to the genus Chlorococcum and 13 to the genus ScenedesmusAir-dry biomass ofmicroalgae ranged from129 to 279 g/l of environment The lipid content of effective strains of Chlorococcum sp4, Chlorococcum sp8, Chlorococcum sp12 and Chlorococcum sp37 was 418 - 580%of the total dry biomass ofmicroalgaeHigh lipid accumulation observed in cells of cultures Scenedesmus sp29 and Scenedesmus sp37- 40,3%and 48,0%, respectively

4 citations


Journal Article
TL;DR: In continuous and semi-continuous cultures, the OD at 435 nm was suitable for estimating cell number and biomass; however, in autotrophic batch cultures an increase of the DW to OD ratio was observed during culture growth for all four algae.

4 citations


Patent
16 Dec 2014
TL;DR: In this paper, the development of chitin and chitosan from microalgae belonging to the phylum Haptophyta to the Phylum Chlorophyta or to the PHylum Heterokontophyta is described.
Abstract: The invention relates to the field of polymer production, in particular to the production of chitin and chitosan from microalgae belonging to the phylum Haptophyta to the phylum Chlorophyta , or to the phylum Heterokontophyta , particularly from microalgae of the genus Isochrysis , Chlorella , Bracteacoccus , Chlorococcum , Scenedesmus , Desmodesmus , Haematococcus , Thalassiosira and Nannochloropsis , as well as to microalgal extracts thereof and their uses.

1 citations


Patent
12 Nov 2014
TL;DR: Wang et al. as mentioned in this paper disclosed high-oleaginous Chlorococcum lianyuanensis and use thereof and used it for producing biodiesel, treating domestic wastewater, producing pigments, fixing carbon dioxide or serving as initial feed in fishery aquaculture.
Abstract: The invention discloses high-oleaginous Chlorococcum lianyuanensis and use thereof. The Chlorococcum lianyuanensis disclosed by the invention is collected by the China general microbiological culture collection center (CGMCC), which is located at Institute of Microbiology, Chinese Academy of Sciences, 3#, 1# Courtyard, Beichen West Road, Chaoyang District, Beijing, on December 6, 2013 and has the collection number of CGMCC No. 8605. The Chlorococcum lianyuanensis can be used for producing biodiesel, treating domestic wastewater, producing pigments, fixing carbon dioxide or serving as initial feed in fishery aquaculture.

01 Jan 2014
TL;DR: The composition of oil produced under phototrophic and heterotrophic growth was significantly diferent, with the former enriched in polyunsaturated fatty acids while the latter contained more of mediumchainsaturatedfatty and monouns saturated fatty acids suitable for biodiesel production.
Abstract: Chlorococcum sp. RAP13—afreshwater 13 microalga was evaluated for growth and lipid production in Q1 14 medium containing 50 % natural sea water under both 15 phototrophic and heterotrophic cultivation modes. The algal 16 cells adapted very well to the sea water and accumulatedupto 17 38 % of their dry cell weight as lipids under heterotrophic 18 cultivation. Biomass production and lipid accumulation were 19 significantlyhigherthanthoseinfreshwater.Supplementation 20 ofbiodieselindustrywasteglycerolinseawaterresultedinthe 21 highest yield of oil (330 mg L −1 ), and the biomass yield was 22 0.85 g L −1 . The composition of oil produced under 23 phototrophic and heterotrophic growth was significantly dif- 24 ferent, with the former enriched in polyunsaturated fatty acids 25 whilethelattercontainedmoreofmediumchainsaturatedfatty 26 acids and monounsaturated fatty acids suitable for biodiesel 27 production.Whiletheuseofseawaterreducestherequirement 28 ofmineralsupplementationandsavesfreshwater,theabilityto