Showing papers on "Color reaction published in 1989"

4-(2-Quinolylazo)phenol as a Spectrophotometric Reagent for Palladium(II), Osmium(VIII), and Gold(III)

Abstract: Reaction behavior of 4-(2-quinolylazo)phenol (p-QAP) is established with three noble metals, i.e. palladium(II), osmium(VIII), and gold(III), under various conditions. Palladium(II) forms a 1:1 greenish violet complex at pH 8–10 (absorption maximum at 580 nm), while osmium(VIII) forms a 1:1 violet compex at pH 8–9.5 (absorption maximum at 550 nm) with the ethanolic solution of p-QAP. Color reaction with gold(III) with ethanolic solution of p-QAP is instantaneous, but fades away with time. However, a 4:4:2 mixture of water, ethanol, and cyclohexanol stabilizes this color for 45 min. Thus a 1:1 red gold(III) complex formed at pH 8–9.5 absorbs maximum at 550 nm. The Sandell’s sensitivities (ng cm−2) of the color reactions are 2.7, 3.9, and 9.8 with palladium(II), osmium(VIII), and gold(III) respectively. Other physicochemical characteristics of the complexes are established. A number of foreign ions tested for their interferences and use of masking agents wherever necessaty it tabulated. Synthetic solutions ...

Automatic analyser and automatic container feed apparatus

Abstract: PURPOSE: To realize a random access system by feeding a reaction tube to a reaction line for every measuring item through a reaction tube feeder. CONSTITUTION: A reaction tube feeder 6 successively feeds reaction tubes 4 of selected measuring items to a reaction line one at a time and the position where the reaction tubes 6 is fed is set as a start point. At first, the reaction tube 4 of an indicated item is inserted in the reaction tube holder of the line 2 from the feeder 6 and a specimen is distributed to the reaction tube 4 in a specimen distribution part 8 while a diluent and an enzyme standard solution are distributed to the reaction tube 4 in a reagent distribution part 10. Thereafter, antigen-antibody reaction is carried out for 43min before reaching a washing mechanism 12 and the reaction tube 4 is washed by the mechanism 12. Further, a substrate solution is distributed in a substrate solution distribution part 14 and color reaction is carried out for 16min until absorbancy is measured in an absorbancy measuring part 16 and absorbancy is measured in the absorbancy measuring part 16. Thereafter, the residual solution is disposed and the reaction tube 4 is discharged. In this one-step method, one analysis is completed within 60min of one round. COPYRIGHT: (C)1991,JPO&Japio

Diazotised 4-Nitroaniline as a Chromogenic Reagent for the Determination of Indole Derivatives in Certain Pharmaceutical Preparations

Abstract: A sensitive colorimetric method has been devised for microdetermination of six indole derivatives; ergotamine tartrate, methylergometrine maleate, dihydroergocornine methanesulphonate, dihydroergocristine methanesulphonate, dihydroergocryptine methanesulphonate and pindolol, both in pure form and in pharmaceutical preparations. The method is based on the reaction of indole moiety with diazotised 4-nitroaniline in buffer solution of pH 6 to produce a stable yellow monoazo dye. Beer's law is obeyed over final concentration ranges 8–32 μgm1−1 for ergotamine tartrate, 4–48 μgml−1 for methylergometrine maleate, 8–56 μgml−1 for dihydro-ergot alkaloids and 1–10 μgml−1 for Pindolol with apparent molar absorptivity range (7.62 × 103−2.61 × 104) 1.mole−1.cm−1. A study has been made to determine the optimum conditions of the colour reaction.

Spectrophotometric Determination of Neomycin With 2,4-Dinitrofluorobenzene

Abstract: A spectrophotometric method is described for the determination of neomycin based on the reaction with 2,4-dinitrofluorobenzene. the best conditions for the reaction were obtained by using 0.02 M borate buffer (pH 9.0) as diluent, under ambient conditions after 50 minutes of reaction.

Novel azoxy compound, its production and microorganism producing same

Abstract: NEW MATERIAL:The compound of formula I (X is group of formula II or III). USE:An antimycotic agent. PREPARATION:The compound of formula I can be produced by culturing a microbial strain KC-7367 belonging to Streptomyces genus and separated from the soil collected in Shimane Prefecture, Japan, separating a filtrate from the cultured liquid by conventional process such as filtration or centrifugal separation and extracting the filtrate with an adsorbent or a solvent. The compound of formula I wherein X is group of formula II is named as KA-7367A substance and the compound of formula I wherein X is group of formula III is named as KA-7367B. The properties of KA-7367A are: nature, neutral colorless oily substance; molecular weight, 198; elemental analysis, C 60.16%, H 9.12%, N 13.98%; molecular formula, C10H18N2O2; solubility, soluble in chloroform, acetone and methanol and insoluble in water; positive to potassium permanganate reaction and negative to ninhydrin reaction. The properties of KA-7367B are: nature, neutral colorless oily substance; molecular weight, 200; elemental analysis, C 59.95%, H 10.13%, N 13.86%; molecular formula, C10H20N2O2; solubility and color reaction, same as those of KA-7367A.

Novel antibiotic r106, production and use thereof

Abstract: NEW MATERIAL:Antibiotic R106 shown by the formula (MeVal is N- methylvaline; NePhe is N-methylphenylalanine; beta-HOMeVal is beta-hydroxy-N- methylvaline) having the following physical and chemical properties. Elemental analysis (%): C 65.0, H 8.5 N 9.9. Melting point: 138-140 deg.C. Specific rotatory power: [alpha] =- 2554.3 (C=1.0 methanol). Molecular weight: FAB-MS m/Z 1,101 (N+H), 1123 (M+Na). Color reaction: positive in 50% sulfuric acid and KMnO4 and negative in ninhydrin and FeCl3. Solubility: soluble in chloroform, methanol, ethanol, DMF and dimethyl sulfoxide and slightly soluble in water. Color: white. Neutral substance, etc. USE:An antimicrobial agent. PREPARATION:A strain of Aureobasidium NoR106 (FERM BP-1938) is cultured.

Novel antibiotic substance kt-6291a and kt-6291b, microorganism capable of producing the same, its production and plant disease injury controlling agent

Abstract: NEW MATERIAL:An antibiotic substance KT-6291A, expressed by the formula and having the following physico-chemical properties. Molecular weight; 882. Composition formula; C 41 H 74 N 19 O 11 . Melting point; gradually browning from 170°C. Specific rotatory power; [α] =+9.4° (C=0.5, methanol). Color; white. Solubility; readily soluble in methanol, butanol and dimethyl sulfoxide and insoluble in ethyl acetate, chloroform and hexane. Color reaction;positive to the Dragendorff reaction, Rydon-Smith reaction and SAKAGUCHI reagent and negative to ninhydrin reaction. Basic, etc. USE: A plant disease injury controlling agent. PREPARATION: A strain, such as Bacillus.sp. KB-291, belonging to the Bacillus is cultured at pH5 to 8 and 18 to 42°C, preferably in a liquid culture medium for 2 to 7 days. COPYRIGHT: (C)1990,JPO&Japio

Antibiotic pk-1061g and pk-1061h and production thereof

Abstract: NEW MATERIAL:A compound shown by formula (R is C12H25 or C13H25) having the following physical and chemical properties. Shape: both white powder. Melting point: both decomposing at >=190 deg.C. Molecular weight: RK-1061G 1036, RK-1061H 1024 (MH ) (FAB mass spectrum). solubility: both readily soluble in water and dimethyl sulfoxide, soluble in methanol and ethanol and insoluble in ethyl acetate and chloroform. Color reaction: both decoloring solution of potassium permanganate, positive in anisaldehyde-sulfuric acid reagent, anthrone reagent, etc. Amphoteric substance. USE:An antimicrobial agent. PREPARATION:A strain such as Streptomyces sp. RK-1061 (FERM-P 8278), belonging to the genus Streptomyces is cultured.

New antibiotic substance sf2197c and production thereof

Abstract: NEW MATERIAL:The antibiotic substance SF2197C expressed by formula and having the following physical and chemical properties. Appearance, colorless acicular crystal; melting point, 54°C; specific rotation, [α] =-37.2° (C=1, methanol); elemental analysis (%), C 59.6, H 9.3, N 11.3; mass spectrometry, molecular ion peak at m/z=440(M + ) by FD-MS; molecular formula, C 22 H 40 N 4 O 5 ; solubility, soluble in methanol, chloroform, acetone and ethyl acetate and insoluble in water and n-hexane; color reaction, positive to Lemieux, iodine reagent and FeCl 3 reagent and negative to ninhydrin reagent; etc. USE: Antibacterial agent. PREPARATION: A microbial strain belonging to genus Actinomadura such as Actinomadura atramentaria (FERM P-7213) is cultured by submerged culture process at 25-32°C for 2-7 days. COPYRIGHT: (C)1991,JPO&Japio

Novel macrolide antibiotic substance mycinamicins and production thereof

Abstract: NEW MATERIAL:The compound of formula (R is H or OH) or its salt. Mycinamicin X has the following physical and chemical properties. Color and appearance, white powder; molecular formula, C42H70N2O15S; elemental analysis (%), C 56.51, H 8.23, N 3.11, S 3.44; molecular weight, 875.08; TLC (silica gel), 0.52; color reaction, positive to decoloration of KMnO4 aqueous solution and negative to ninhydrin reaction, Sakaguchi's reaction and FeCl3 reaction; solubility, soluble in water and alcohol such as methanol, hardly soluble in acetone and insoluble in ethyl acetate, benzene, etc.; acidity or basicity, ampholytic; etc. USE:Antibacterial agent. PREPARATION:The objective compound can be produced by culturing a microbial strain belonging to Micromonospora genus and capable of producing mycinamicins [e.g. Micromonospora griseorubida A11725 (FERM BP-705)].

Studies on the Mimetic Active Centre of Peroxidase and Catalytic Color Reaction

Abstract: The chelate compound of manganese tetra-(4-sulphophenyl) porphine (Mn-TPPS4) has been synthesized. The catalytic characteristics of Mn-TPPS4 as mimesis of peroxi-dase on chromogenic reaction of hydrogen peroxide with 4-aminoantipyrine and phenol have been studied. Trace of hydrogen peroxide can be determined with accuracy and precision. The glucose in human serum has been determined by the proposed reaction combining with catalytic reaction of glucose oxidase with satisfactory results.

Spectrophotometric determination of anionic surfactants in environmental water samples with ethyl violet

Abstract: A highly sensitive and direct Spectrophotometric method for determination of anionic surfactants in water was developed. The optimal conditions for the colour reaction of the ion-associate of anionic surfactant with ethyl violet in the presence of polyvinyl alcohol was described. The suitable pH range is between 2.2 and 2.8 with C8H5O4K-HC1 buffer solution. The wavelength of maximum absorbance of the ion-associates is at 550nm and the molar absorptivity are 1.99×105 L.mol-1.cm-1 for dodecylbenzene sulfonate, 1.93×105 L.mol-1.cm-1 for dodecylsulfate, 1.07×105 L.mol-1.cm-1 for dodecylsulfonate, respectively. The method has high sensitivity and fairly good selectivity and so it can be applied to the Spectrophotometric determination of micro amounts of anionic surfactants in environmemtal water samples.

Novel polymeric polysaccharide f-b

Abstract: NEW MATERIAL:Polymeric polysaccharide F-B. Element analysis (%): F-B standard sample salt C 34.8, H 5.5, N 0.6, ash 11.6, F-B standard sample C 37.1, H 5.9, N 0.5, ash 5.2; range of molecular weight (gel filtration method); 7,000-17,000; average molecular weight (gel filtration method): 12,000; solubility: soluble in water, etc., insoluble in methanol, etc.; color reaction: positive to phenol sulfuric acid reaction, etc., negative to Ninhydrin reaction, etc.; color: white; constituted sugar and said composition: glucose:glucuronic acid=3.4:1; distinction of basic, acidic or neutral: 0.1% aqueous solution is acidic. USE:Anti-plant virus agent. Producible in large quantities, in a low cost and safety in fermentation industrially. PREPARATION:A production fungus of polymeric polysaccharide F-B belonging to Fomes fomentarius [preferably Fomes fomentarius JTS 3046 (FERM-P 9704)] is cultured and the aimed composition is collected from the cultured material.

Phthalide compound capable of color reaction and recording material with said compound as color forming component

Abstract: PURPOSE:To enable images to be satisfactorily read by an optical character reader or an image reader, by using a specified phthalide compound capable of performing color reaction and having strong absorption in a near infrared region at or above a specified wavelength. CONSTITUTION:A phthalide compound of formula I, wherein R is hydrogen or an alkyl, A is hydrogen, an alkyl or halogen, and B is hydrogen or a substd. amino, is a white or pale yellow crystalline powder, shows strong absorption in a near infrared region at or above 700nm, and performs a color reaction to give a blue - green color or other color upon making contact with a color developer. A color reaction agent to be used in combination with the leuco dye may be an electron-acceptive compound, an oxidizing agent or the like. In the case of producing a pressure-sensitive recording material, a color reaction agent sheet is produced by dispersing or dissolving the color reaction agent in water or an organic solvent by using an appropriate dispersing agent, with optional addition of a binder, and applying the resultant liquid to a base such as a paper, whereas a color developer sheet is produced by dispersing the leuco dye in a microencapsulated state by using an appropriate dispersing agent, and applying the resultant liquid to a base such as a paper. On the other hand, in the case of producing a thermal recording material, liquids separately prepared by respectively dispersing the leuco dye and the color reaction agent are mixed with an appropriate binder, and the resultant mixed liquid is applied to a base such as a paper.

Photometric determination of 2,2-isopropylidenebis-phenol (dian) by reaction with nitrous acid: Identification of the chromogen

Abstract: Optimum conditions for the colour reaction of dian with nitrous acid in acid medium followed by alkalization with ammonia have been found. Investigation showed that 2,2′-dinitrodian is the main product of the reaction, accompanied by a small amount of 2,2′,6-trinitrodian. The ammonium salts of those compounds are responsible for the colour.