Showing papers on "Genomics published in 1980"
TL;DR: Analysis of sequence organization in the genome of animals from different phylogenetic groups points at two major types of DNA sequence organization, the Xenopus and the Drosophila type, named after the first objects in which these types of genome arrangement were revealed.
Abstract: Analysis of sequence organization in the genome of animals from different phylogenetic groups is one of the approaches to study the molecular mechanism of genome evolution. This analysis is based, in particular, on the study of interspersion of repetitive DNA sequences with unique ones by reassociation of DNA fragments of different lengths. To separate fragments with duplexes from single-stranded fragments, hydroxyapatite is used (Britten et al., 1974; Davidson et al., 1975; Galau et al., 1976). Information obtained for the genomes of animals and plants points at two major types of DNA sequence organization, the Xenopus and the Drosophila type, named after the first objects in which these types of genome arrangement were revealed. The Xenopus type is characterized by short repetitive sequences (0.2-0.6 kb*) interspersed with short unique sequences (0.8-2 kb) on a rather small DNA stretch (0.9-2.5 kb). The Drosophila type DNA arrangement is found to be an alteration of very long (up to 5 kb) repetitive sequences with unique sequences longer than 10 kb. Both types of interspersion are supported by electron microscopy studies (Manning et al., 1975; Chambedin et al., 1975). The Drosophila type of DNA sequence reported for three insect species has been recently found in the genome of Gallus domesticus (Epplen et al., 1978; Ginatulin et al., 1979).
5 citations