Showing papers on "Metabolome published in 2000"

“OMICS” | CE and CE-MS for Metabonomics/Metabolomics

Abstract: One particular field where analytical measurement science has played a key role is in what sometimes has been denominated the ‘third’ level of functional genomic analysis or metabolome analysis (commonly referred to as metabolomics or metabonomics). In particular, developments in technology for profiling complex matrices such as biofluids, environmental samples, or natural matrices have certainly increased in recent years. The major challenges to overcome when measuring the metabolome are its chemical complexity and heterogeneity of metabolites, and the wide dynamic range of these biochemical species. Capillary electrophoresis (CE) is proving to be a truly useful addition in the metabolomics analytical toolbox especially as it has high-throughput potential, enabling rapid screening of metabolite classes as well as metabolic fingerprinting. CE with ultraviolet (UV) detection could in fact be viewed as a first-stage metabolomics tool – especially useful for clinical and natural product laboratories. Meanwhile, CE-mass spectrometry (MS) is a more sophisticated but more expensive tool. CE confers high resolving power and separating speed to the analysis, MS provides high selectivity and structural elucidation ability. The major advantages of combined CE-MS are an extremely high resolution and that almost any charged species can be infused into MS. The state of the art in CE and CE-MS applications in metabonomics/metabolomics are discussed in this article.