Showing papers on "Mimosine published in 2023"
TL;DR: In this paper , the effects of L-mimosine on immune responses in Wistar rats were evaluated and it was found that Limosines did not compromise macrophage activity and inhibited T-dependent clonal expansion during the immune response.
TL;DR: In this paper , the authors explored the extraction method of mimosine, pharmacological study and phytochemical study in Leucaena leucocephala trees and reported the presence of various secondary metabolites such as alkaloid, cardiac glycosides, tannins, flavonoids, saponins, and triterpenoids.
Abstract: Mimosine [α-amino-β-(3-hydroxy-4-oxo-1, 4- dihydropyridin-1-yl)-propanoic acid] is toxic element, that found naturally in Leucaena (Leucaena-leucocephala de Wit) tree. Leucaena leucocephala trees are commonly known as White Lead tree. It has been used for medicinal purposes because of possessing multiple pharmacological properties. Studies have shown the presence of various secondary metabolites such as alkaloid, cardiac glycosides, tannins, flavonoids, saponins, and glycosides in this species. Phytochemically, this plant has been reported to contain numerous alkaloids, flavonoids, saponins, tannins, and triterpenoids. Pharmacologically this plant has been reported as antibacterial, antidiabetic, anti-inflammatory, anticancer, anthelmintic, antioxidant. In the present review article is to explore extraction method of mimosine, pharmacological study and phytochemical study.
14 Jun 2023
TL;DR: In this paper , the anti-melanoma effect of L-mimosine was evaluated using MTT, plate colony formation (PCS), and flow cytometry analysis, and the results showed that L-minimosa inhibited melanoma cells (A375 and B16) proliferation by increasing the apoptosis rate.
Abstract: Abstract Clinical data have shown that emerging therapies are still not effective in controlling melanoma growth. L-mimosine is known to fight against the growth of various tumors, but the in vitro and in vivo studies of L-mimosine against melanoma are still lacking in detailed studies. In this study, we used MTT assay, plate colony formation assay, and flow cytometry analysis to evaluate the anti-melanoma effect of L-mimosine. The results showed that L-mimosine inhibited melanoma cells (A375 and B16) proliferation by increasing the apoptosis rate. In a mechanistic study, we found that L-mimosine obviously decreased the permeability of mitochondrial membrane by downregulating Bax and upregulating Bcl-2, after which it significantly increased the levels of reactive oxygen species, cytoplasmic cytochrome C, cleaved caspase 9, and cleaved caspase 3, leading to melanoma cell apoptosis. Furthermore, we found that antioxidant NAC obviously counteracted the inhibitory effect of L-mimosine on melanoma cell growth in vitro and in vivo . The results suggested that L-mimosine induced melanoma cell apoptosis through a reactive oxygen species–dependent mitochondrial apoptotic pathway. The results of this study will provide some data and theoretical support for the research on the anti-melanoma mechanism of L-mimosine.