Showing papers on "Murashige and Skoog medium published in 1970"

In vitro fertilization in the self-incompatible plant, Petunia hybrida

Abstract: The possibility to obtain the seeds by the method of test-tube fertilization was examined using a self-incompatible plant, Petunia hybrida, and a self-compatible one, Eschscholtzia californica, as a control.1. Pollen grains from both tested plants germinated and grew well on the sucrose-borate medium. On the basal medium (Table 1), pollens of E. californica germinated considerably, while those of P. hybrida hardly germinated. Pollen germination and tube growth of the latter were improved, however, in following two methods; (a)“style culture”or (b) culture of whole mass of bare ovules with placental tissue dusted with pollen grains.2. With E. californica, the ovules developed normally at the early period of the culture, but well developed seeds were not obtained in spite of the subculturing. Some of well swelled ovules formed callus having bud primordia like protuberances.With. P. hybrida ovules developed well by culturing the whole mass of bare ovules with pollen grains in both cross (fertile) or self (sterile) combinations. Some of the seedlings were obtained by subculturing the developed seeds onto basal medium in the former combination and also in the latter by employing MURASHIGE and SKOOG medium.

Micropropagation of Isoplexis chalcantha Svent, O´Shanahan from Mature Plants

Abstract: Culture medium requirements for micropropagation of Isoplexis chalcantha was achieved for the first time after high degree of contamination and phenolic exudates were detected and solved. Cultures were established from axillary shoots using juvenile branches collected from this medicinal plant. Most satisfying results were obtained using a solidified and a modified MS medium (NO 3 - : NH 4 + ratios) enriched with ascorbic acid or soluble PVP plus GA 3 , BAP and NAA. Explants (nodal segments) were used for in vitro shoots multiplication and best results were achieved with modified MS plus BAP and auxins. Vigorous shoots rooted without symptoms in the half-strength modified MS enriched with low concentration of IBA. Key words: Isoplexis chalcantha, a xillary shoots, c ontamination, p henolic exudates, c ulture media, NO 3 - : NH 4 + ratios D.O.I. 10.3329/ptcb.v18i2.3395 Plant Tissue Cult. & Biotech. 18 (2): 131-137, 2008 (December)

Potential Embryogenic Callus Induction Protocol Through Cell Suspension Culture For High Frequency Plant Regeneration Of Maspine Pineapple (Ananas comosus L.)

Abstract: To explore the potential for embryogenic callus induction protocol through cell suspension culture forhigh frequency plant regeneration of Maspine pineapple (Ananas comosus L.), eight different culturemedia formulation were evaluated for their effects on the induction of somatic embryos from suckerexplants. Explants were cultured on MS medium supplemented with various media concentration(NAA, Dicamba and BAP, Picloram, Kinetin and NAA, 2,4-D, TDZ, and TDZ and BAP).Embryogenic callus induction percentage, color and texture of the callus were assessed after fivemonths of culture. The optimum medium for the proliferation of in vitro shoots from sucker explantswas MS medium supplemented with 3 mg/L BAP. Meanwhile, the optimum medium for the inductionof fastest and high percentage of embryogenic callus growth from in vitro leaf-based was MS mediumsupplemented with Picloram. Results of mean comparison showed that 3 mg/L Picloram were moreeffective on explants than 10 mg/L. Results of the double staining method proved that somaticembryogenesis occurred in MS supplemented with 3 mg/L Picloram. Under microscopic observations,the globular-stage of the embryos were revealed in callus cells which is relatively suitable forsuspension cells inoculums, indicating that the tested PGR were significantly effective for somaticembryogenesis formation in this species. Most embryogenic callus from sucker explants wasyellowish-mucilaginous-wet-friable. The developed protocol potentially leads to the production ofembryogenic callus from sucker explants and plant regeneration through somatic embryogenesis.