Showing papers on "Pathogenic bacteria published in 1980"

Enumeration of potentially pathogenic bacteria from sewage sludges.

Abstract: To ascertain the health risks that may be posed by the land application of sewage sludges, a scheme was devised to determine the types and numbers of pathogenic and potentially pathogenic bacteria present in sludges. A processing treatment was adapted to sludge to give a homogenate which yielded the greatest numbers of viable bacteria. Conventional methods were successful in enumerating Klebsiella, Staphylococcus, gram-negative enteric bacteria, and commonly used indicator organisms. Modifications of conventional methods improved the enumeration of Salmonella, Mycobacterium sp., fluorescent Pseudomonas sp., and Clostridium perfringens. However, Shigella methodology yielded only one isolate. Utilizing the proposed scheme, the population densities of these organisms were estimated in three domestic wastewater sludges. In light of these results, the potential impact of land application of sewage sludges is discussed.

Method for sewage treatment with bacteria.

Abstract: A process for the treatment of sewage wherein the sludge is inoculated with a bacteria, L. plantarum, and a carbohydrate such as lactose is admixed therewith. The addition of the bacteria and the carbohydrate without more, drops the pH of the sludge to below 4.0. This results in the elimination of pathogenic bacteria and renders the sludge suitable for use as a soil extender without any further environmental constraints.

Factors affecting the differential counting of human lymphocyte subpopulations in blood smears.

Abstract: We have previously used the antibody-mediated binding of bacteria to identify Ig-bearing cells and the natural binding of bacteria to identify several lymphocyte subpopulations. By using bacteria this identification can be carried out in conventional blood smears since bacteria are small and easily distinguished from all blood elements. To develop a standardized method for identification of lymphocyte subpopulations that may become useful in clinical laboratories, we investigated here several parameters that might affect the safety and accuracy of the test, and that might simplify the procedure. We found that: (1) the rosettes formed between bacteria and lymphocytes cannot be disrupted by vigorous handling; (2) the ability fo form rosettes is a stable property of the bacterial strains; (3) for optimal results the blood sample must not be stored for more than 4 hr at 25 degrees C and the entire procedure must be performed in medium supplemented with 6% bovine serum albumin; (4) a large excess of anti-Ig antibody is required for the optimal coating of bacteria to detect Ig-bearing cells; (5) both the antibody-coated bacteria and formaldehyde-fixed bacteria can be stored at 4 degrees C or at -20 degrees C for at least 6 months; (6) the buffy coat from the blood sample can be used instead of the whole blood to reduce the time required for reading the smears; and (7) some of the pathogenic bacteria can be killed by autoclaving without modifying their binding properties. A complete and simple method which uses the bacterial adherence for the identification of lymphocyte subpopulations in blood smears is presented.

Bacteriological findings in the cervical mucus of sterile women

Abstract: In 61 sterile women the cervical secretion was examined bacteriologically. Neisseria gonorrhoeae was found in one women (1.6%), in two women the nutrient media remained sterile, in three women no pathogenic bacteria were observed, while in the remaining 55 women (90.16%) there were microorganisms without any pathogenic effect on the cervix. The kind and percentage of the isolated bacteria are shown in tabular form.

[Epidemiology and bacteria in infectious sinus diseases (author's transl)].

Abstract: A number of studies show that only a few bacteria are dominating the infectious sinus diseases In acute sinusitis hemophilus and pneumococci are prevalent Recent results in chronic sinusitis indicate a greater importance of anaerobic bacteria The bacteriological findings in sinusitis are influenced by many factors: 1 the sampling techniques (proof-puncture or nasal-swab); 2 transport time and transport medium; 3 bacteriological culture technique; 4 the isolated bacteria are not automatically pathogens The difference between colonizing or contaminating bacteria and pathogenic bacteria should be taken into account A lack of leukocytes, a mixture of bacteria and a viable count of 10(3) or lower indicates normal flora or contamination; 5 kind of infection: acut - chronic; dentogen - rhinogen; 6 mode and duration of therapy (antibiotics, surgery, irrigation); 7 age of patient, season of the year

[Clinical studies on cefoperazone in the pediatric field (author's transl)].

Abstract: Cefoperazone (CPZ) at dose levels of 80 approximately 100 mg/kg/day, divided 3 approximately 4 times, was drip-infused or intravenously injected for a period of 2 approximately 6 days to 10 patients. All 10 cases, 4 cases of bronchopneumonia from which H. influenzae was detected (Group A S. pyogenes and S. pneumoniae were also detected in each 1 case). 2 cases of coli urinary tract infection, 1 case of acute colitis from which pathogenic E. coli was detected, 1 case of E. coli carrier, 1 case of acute bronchitis (bacteria were not detected), and 1 case of urinary tract infection (bacteria were not detected) showed rapid improvement of clinical symptoms with rapid eradication of the pathogenic bacteria. In one case of urinary tract infection where S. epidermidis and S. faecalis were simultaneously detected, S. epidermidis was removed but S. faecalis was merely decreased. The effective antibacterial concentration after intravenous injection of CPZ in the feces was determined and found to be present in sufficient concentrations to prevent colon infection. No particular side effects were observed during CPZ therapy.