Showing papers on "Penicillium griseofulvum published in 2014"

Determination of the effects of initial glucose on the production of α-amylase from Penicillium sp. under solid-state and submerged fermentation.

Abstract: The effects of catabolite repression of initial glucose on the synthesis of α-amylase from Penicillium chrysogenum and Penicillium griseofulvum were investigated under solid-state fermentation (SSF) and submerged fermentation (SmF) systems. The results obtained from either fermentation were compared with each other. In the SmF system, initial glucose concentration above 10 mg/mL completely repressed the production of α-amylase from P. chrysogenum and P. griseofulvum. However, the repression in the SSF system was not complete, even when the glucose level was raised to 160 mg/g.

Screening of Lactic Acid Bacteria for Antifungal Activity against Fungi

Abstract: Many chemical preservatives that target fungi growth in food have been approved and used for many years. Recently the consumers are looking and demanding for products without chemical preservatives and still maintain good shelf life and safe. The growth of spoilage fungi have been a global concern because of the economy loses and the health hazard of the mycotoxins produced by the spoilage fungi. A total of 22 lactic acid bacteria isolated from Tarhana and Lactic acid bacteria were screened for antifungal activity using dual agar overlay method and well method against Alternaria alternata, Aspergillus parasiticus, Aspergillus oryzae Penicillium griseofulvum, Penicillium chrysogenum, Penicillium notatu, Penicillium citrinum, Penicillium roquefort, Aspergillus fumigatus. Ten isolates showed inhibition activity after 72 h incubation at 30°C. Supernatant of 10 isolates with strong antifungal activity was evaluated by well method and they inhibited the growth of the fungi at 30°C for 72 h. F2,1 supernatant reduced the mass growth of Penicillium griseofulvum, Penicillium chrysogenum Aspergillus fumigatus and Aspergillus parasiticus when incubated for 6 days at 30°C. The isolates were identified using rapid ID 32 Strep as Enterococcus durans F2.1. F2.1 isolates studied inhibited the growth of the mycelia and conidia germination of the fungi which indicate the possibility of using LAB isolates as biopreservative.

Screening, molecular characterization and phylogenetic analysis of Penicillium sp producing high levels of protease

Abstract: This paper deals with the isolation, screening, molecular characterization and phylogenetic analysis of proteolytic fungi. Forty seven fungal isolates were preliminarily screened for their ability to produce protease on skimmed milk agar. Among the forty seven isolates, thirty four isolates were protease positive. Based on the secondary screening, three promising proteolytic fungi were selected for further investigations. The DNA was extracted from the fungi, ITS-rDNA amplified by PCR and its ITS regions were sequenced. Based on the molecular characterization and phylogenetic studies, the selected isolates were identified as Penicillium citrinum LCJ 222 (Accession no. KF414682), Penicillium griseofulvum LCJ 231 (Accession no. KF414683) and Penicillium citrinum LCJ 233 (Accession no. KF414684).