Showing papers on "Personal genomics published in 1995"
TL;DR: There is a change in immediate emphasis in the human Genome Project from production of detailed genome maps intended to guide researchers to their goal to actual sequencing of genes on chromosomes.
Abstract: This article reports on a change in immediate emphasis in the human Genome Project from production of detailed genome maps intended to guide researchers to their goal to actual sequencing of genes on chromosomes. The politics, cost, and different approaches are all discussed.
23 citations
TL;DR: Two completing technologies based on these two different targets are discussing in general in this article.
Abstract: Since the bigging of the Human Genome Project, the focus has been mainly on developing the physical and genetic maps needed to guide the sequencing effort. However this has begun to change with the suggestion that the human genome could be completely sequenced, using technology already available, as early as the year 2001 at the cost of 10-12 cents per base if the sequencing accuracy were dropped from the original target of 99.9% accuracy to 99.90% accuracy. Two completing technologies based on these two different targets are discussing in general in this article.
12 citations
TL;DR: The resulting approach has since been used to produce almost all of the genome sequencing data for C. elegans and will, with appropriate modifications, continue to be the strategy of choice for the completion of this genome sequence and the beginning of the human genome.
Abstract: High-throughput DNA sequencing of the C. elegans genome currently is ongoing at our Genome Sequencing Center (GSC) at Washington University School of Medicine in St. Louis and at the Sanger Centre in Hinxton, England. Our collaborative effort is aimed at completion of this 100 Mb genome by the end of 1998. Now just into our fifth year of sequencing, we have amassed a combined output of over 22 Mb of completed sequence. The first two years of the pilot project were spent investigating and evaluating different approaches to genome sequencing with respect to throughput, ease of associated techniques, and methods and their robustness. The resulting approach has since been used to produce almost all of the genome sequencing data for C. elegans and will, with appropriate modifications, continue to be our strategy of choice for the completion of this genome sequence and the beginning of the human genome. The basic strategy consists of three phases: a library construction phase, a production sequencing ("shotgun") phase, and a directed "finishing" phase. >
3 citations
TL;DR: Major accomplishments of the HGP are summarized briefly including maps of at least two chromosomes, 16 and 19, are in the final difficult stage and will be the first to be completely sequenced; evidence is being refined on the myotonic dystrophy gen; and an attempt to fashion a silicon chip to detect specific DNA sequences.
Abstract: This is a report on the Genome Mapping and Sequencing meeting from 10-14 May 1995. Debate included how to start the final stage of the Human Genome Project (HGP) - large scale sequencing and the problem of funding. Major accomplishments of the HGP are summarized briefly including: maps of at least two chromosomes, 16 and 19, are in the final difficult stage and will be the first to be completely sequenced; evidence is being refined on the myotonic dystrophy gen; and an attempt to fashion a silicon chip to detect specific DNA sequences.
3 citations