Showing papers on "Piperlonguminine published in 2019"

Antibacterial, anthelmintic, and analgesic activities of Piper sylvaticum (Roxb.) leaves and in silico molecular docking and PASS prediction studies of its isolated compounds

Abstract: Background In the present study, we investigated the antibacterial, anthelmintic, and analgesic activities of methanol extract of P. sylvaticum leaves (MEPSL) in experimental models. Then, computational analysis (in silico molecular docking and PASS prediction) was performed to determine the potent phytoconstituents of total six isolated compounds of this plant for antibacterial and anthelmintic activities. Methods Qualitative and quantitative phytochemical studies were carried out by established methods. In vitro antibacterial activity was determined by disc diffusion technique and anthelmintic activity was tested against Tubifex tubifex worm whereas analgesic activity was determined by the acetic acid-induced writhing test in mice. Molecular docking study was performed using Schrodinger Maestro 10.1 and an online tool used for PASS prediction. Results Our phytochemical study revealed the presence of alkaloids, flavonoids, saponins, and also indicated a substantial amount of phenols (65.83 mg), flavonoids (102.56 mg), and condensed tannins (89.32 mg). MEPSL showed good antibacterial activity against both gram-positive and gram-negative bacteria. Our result exhibited that MEPSL has strong anthelmintic action compared to standard levamisole. In addition, the extract also showed a dose-dependent and statistically significant analgesic activity at the doses of 200 and 400 mg/kg, body weight. Docking studies showed that piperine and piperlonguminine have the best scores for the tested enzymes. PASS predicted the antibacterial and anthelmintic activity of both phytoconstituents. Conclusions This study suggests that MEPSL possess significant antibacterial, anthelmintic, and analgesic activities which could be related to the presence of several phytochemicals. The phytoconstituents, i.e. piperine and piperlonguminine were found to be most effective in computational studies.

Phytotoxic property of Piper retrofractum fruit extracts and compounds against the germination and seedling growth of weeds

Abstract: The fruits of Piper retrofractum Vahl. contain a variety of compounds, but no phytotoxic compound with plant growth inhibitory activity has been reported. Therefore, the phytotoxic activity of P. retrofractum fruit extracts was investigated and found the reduction of seedling growth of cress, lettuce, alfalfa, barnyard grass, Italian ryegrass, and jungle rice. Seven bioactive compounds were isolated from the fruit extracts and identified as 3-phenylpropanoic acid (1), (2E,4E)-methyl piperate (2), (2E,4Z)-methyl piperate (3), piperlonguminine (4), dihydropiperine (5), isochavicine (6), and piperine (7). There have been no reports for their phytotoxic activity. The activity of these compounds was determined against the germination and seedling growth of cress and barnyard grass. The total germination percentage (GP) of cress was significantly inhibited by 1, 2, and 6 (40–100% inhibition), while its germination index or progressive total of cumulative germination (GI) was the most sensitive to 1, leading to the longest time required for 50% germination (T50) at 57 h behind control. However, the seedling growth of cress was highly inhibited by 6 with I50 values of 11 µM. The compounds 1 and 5–7 showed significant inhibition on GP of barnyard grass by 55–47%, while 5 and 1 effectively reduced GI and extended T50 to 42–48 h behind control, and 7 showed the most growth inhibition with I50 values of 37 µM. The inhibitory effects of the seven compounds indicate the phytotoxic property of P. retrofractum fruits, which 1, 5–7 may be the main compounds responsible for the phytotoxicity.

Application of piperlongumine alkaloids in preparation of medicines for promoting melanogenesis during treatment of leucoderma

Abstract: The invention relates to application of piperlongumine alkaloids in preparation of medicines for promoting melanogenesis during treatment of leucoderma. The amide alkaloid compounds include isopiperine, piperlonguminine, pseudocapsaicin amide A and pseudocapsaicin amide C, and are subjected to determination of melanogenesis in murine melanoma cells B16 and tyrosinase activity. The results show that, the piperlongumine alkaloids are capable of promoting melanogenesis and tyrosinase activity to different degrees, and can be used for preparing medicines for treating the leucoderma. A favorable material basis is provided for developing medicines for treating leucoderma.

Evaluation of chemo-diversity in major Piper species for three piperamides using validated RP-HPLC method

Abstract: India boasts rich biodiversity of plants belongs to Piperaceae. These plants are store-house of biologically active alkaloids, generally termed as piperamides. Among piperamides, piperine, piperlongumine and piperlonguminine plays an effective role in pharmaceutical applications. The present study was aimed at developing and validating a simple reverse phase high performance liquid chromatography method to analyze chemo-diversity of these three piperamides in Pipernigrum L., Piper longum L., Piper thomsonii Hook, Piper sarmentosum Roxb. and Piper chaba Trel. & Yunck. The separation was achieved on a RP-C18 column with water (0.1% Acetic acid) and methanol as mobile phase with detection wavelength of 338 nm. The compounds were identified using authentic standards. The developed method was validated as per ICH guidelines for linearity, accuracy, precision, limit of detection and limit of quantification. The method was successfully applied in quantification of these piperamides in berries of five different Piper species. Among them, piperlongumine was detected only in P. longum and P. sarmentosum. Piperlonguminine and piperine was higher in P. chaba and P. nigrum. In conclusion, the method developed could be used for chemo-diversity analysis of different Piper spp to select alternate Piper species for pharmacological preparations based on the composition of its bio-active compounds.