Showing papers on "Restriction map published in 1972"
TL;DR: Sites of restriction and modification by E. coli enzymes, including K restriction enzyme, Hemophilus endonuclease sites, and the role of SAM and ATP in restriction are investigated.
Abstract: RESTRICTION ENDONUCLEASES ... . Restriction endonuclease K . . . . Restriction endonuclease B ... . Restriction endonuclease P .... , . R/ and Rll restriction endonucleases, .. . Hemophilus injiuenzae endonuclease .. , .... . THE ROLES OF SAM AND ATP IN RESTRICTION, , .. SAM." ... . . . .. . . . . . . . , . ATP .. " ,.,., ... . .. , . , . . . . .. , ... . MODIFICATION METHYLASES ... . Modification methylase B, .. . . . ..... , , , . . . Modification methylase P, . . . . .. ,. " ,. " " , .. " .... . ... .. . Modification methylase activity of the K restriction enzyme. , . . . . . . . . . . . . . . . Modification methylase activity of the PI restriction enzyme .. . An RJ modification activity . GENETIC ANALYSIS ., , , , . , . . . . . . . ....... . . ......... , Restrictionless mutants .. , .. Genetic mapping "."" , . . Complementation studies .... . . . . . . . ....... . MODIFICATION AND RESTRICTION SITES ON DNA . . . . . , . , . , . , , , , .. . Hemophilus endonuclease sites. , , , , , , , , , . . . . . . . . . .... . ..... . Sites of restriction and modification by E. coli enzymes . A speculation. . . . . . . . . . . . . . . . . . . . . . .. .
267 citations
TL;DR: The R(1) restriction endonuclease of Escherichia coli converts covalently-closed circular Simian Virus 40 (SV40) DNA to unit-length linear duplex molecules.
Abstract: The R(1) restriction endonuclease of Escherichia coli converts covalently-closed circular Simian Virus 40 (SV40) DNA to unit-length linear duplex molecules. Cleavage occurs at a unique site, since denaturation and renaturation of these linear molecules yield linear but no circular molecules. The distance from the cleavage site to the SV40 DNA sequence contained in the adenovirus-SV40 hybrid, Ad2(+)ND(1), is 0.11 of the length of SV40 DNA. T4 gene 32 protein binds to SV40 DNA in a region 0.45 of the length of SV40 DNA from the R(1) cleavage site. E. coli B restriction endonuclease can cleave SV40 DNA at several sites.
144 citations
TL;DR: The technique presented is intended to provide a general scheme for restricted in vitro RNA synthesis of viral DNA molecules, and appears, by competition-hybridization experiments, to be similar to in vivo RNA extracted from SV40 virus-infected cells.
1 citations