Showing papers on "Sequence assembly published in 1979"
TL;DR: Computer programs that can be used to order both sequence gel readings and clones are described and a method of coding for uncertainties in gel readings is described.
Abstract: With modern fast sequencing techniques and suitable computer programs it is now possible to sequence whole genomes without the need of restriction maps. This paper describes computer programs that can be used to order both sequence gel readings and clones. A method of coding for uncertainties in gel readings is described. These programs are available on request.
355 citations
TL;DR: A collection of user-interactive computer programs is described which aid in the assembly of DNA sequences by searching for the positions of overlapping common nucleotide sequences within the blocks of sequence obtained as primary data.
Abstract: A collection of user-interactive computer programs is described which aid in the assembly of DNA sequences. This is achieved by searching for the positions of overlapping common nucleotide sequences within the blocks of sequence obtained as primary data. Such overlapping segments are then melded into one continuous string of nucleotides. Strategies for determining the accuracy of the sequence being analyzed and reducing the error rate resulting from the manual manipulation of sequence data are discussed. Sequences mapping from 97.3 to 100% of the Ad2 virus genome were used to demonstrate the performance of these programs.
59 citations
01 Jan 1979
TL;DR: In his book Biochemistry Lehninger (1970) illustrated the complexity of the genetic material by devoting a full page to closely spaced arrays of A, G, C, and T letters, calling this “an imaginary base sequence for the chromosome of bacteriophage oX174" and pointing out that this is one of the smallest DNA genomes known.
Abstract: In his book Biochemistry Lehninger (1970) illustrated the complexity of the genetic material by devoting a full page to closely spaced arrays of A, G, C, and T letters, calling this “an imaginary base sequence for the chromosome of bacteriophage oX174” and pointing out that this is one of the smallest DNA genomes known. There has been a remarkable revolution in DNA sequencing technology since that page was composed. Not only has the real nucleotide sequence of oX174 been published (Sanger et al., 1977 a), but also DNA sequences of bacteriophages G4 (Godson et al., 1978) and fd (Schaller et al., 1978) and an animal virus, SV40 (Reddy et al., 1978a; Fiers et al., 1978) are completed, or nearly so, and a remarkable array of DNA sequences from various sources is fast accumulating.
3 citations