Showing papers on "Trichoderma longibrachiatum published in 1995"
Patent•
24 May 1995TL;DR: In this paper, the cloning and high level expression of novel truncated cellulase proteins or derivatives thereof in the filamentous fungus Trichoderma longibrachiatum were discussed.
Abstract: The present invention relates to the cloning and high level expression of novel truncated cellulase proteins or derivatives thereof in the filamentous fungus Trichoderma longibrachiatum. Further aspects of the present invention relate to fungal transformants that express the novel truncated cellulases and derivatives, and expression vectors comprising the DNA gene fragments or variants thereof that code for the truncated cellulases derived from Trichoderma longibrachiatum using genetic engineering techniques.
141 citations
Journal Article•
115 citations
Patent•
16 Aug 1995TL;DR: In this article, an enzyme feed additive is provided comprising a xylanase, a protease, and optionally a β-glucanase, where the ratio of the units of x-layer activity per unit amount of the feed additive to β-layer activation is 1:0-0.25.
Abstract: An enzyme feed additive is provided comprising a xylanase, a protease, and optionally a β-glucanase. The ratio of the units of xylanase activity per unit amount of the feed additive to the units of β-glucanase activity per same unit amount of the feed additive is 1:0-0.25. Preferably, the xylanase is the low pI xylanase and/or the high pI xylanase obtained from Trichoderma longibrachiatum. Preferably, the protease is a mutant subtilisin comprising a substitution at the amino acid residue position equivalent to tyr+217 of Bacillus amyloliquefaciens subtilisin with leucine.
80 citations
TL;DR: A case of brain abscess due to Trichoderma longibrachiatum in a leukemic patient with prolonged neutropenia is reported and definitive cure was achieved after neurosurgical resection of the abscess and prolonged antifungal therapy.
Abstract: A case of brain abscess due toTrichoderma longibrachiatum in a leukemic patient with prolonged neutropenia is reported Definitive cure was achieved after neurosurgical resection of the abscess and prolonged antifungal therapyTrichoderma is a filamentous fungus species, which is only exceptionally pathogenic in humans This genus and particularly the speciesTrichoderma longibrachiatum should be added to the growing list of fungi causing infection in immunocompromised patients
48 citations
40 citations
TL;DR: The results suggest that supplementary enzymes survive the potential hazards of the gastrointestinal tract in sufficient quantity to improve performance in broiler chicks fed barley based diets.
27 citations
Journal Article•
TL;DR: The 29 kDa xylanase from T. harzianum was the most distinct enzyme and was the best performer in the hydrolysis of four out of the five substrates, and the deacetylation of xylan seemed to be able to increase the Hydrolysis yields obtained with the first three xylanases.
Abstract: Two β-1,4-xylanases from Trichoderma harzianum and two from T. reesei were purified to apparent electrophoretic homogeneity using a combination of cation-exchange, anion-exchange, hydrophobic-interaction and gel-filtration chromatography. Five different substrates, representing different forms of xylan, were isolated from either the hardwood Populus tremuloides (trembling aspen) or the softwood Pinus contorta (lodgepole pine). After incubating these substrates with different loadings of each enzyme, hydrolysis yields of reducing sugars were quantified. Statistical analyses highlighted differences among the xylanases. The 20 kDa alkaline xylanase from T. harzianum and that from T. reesei showed very similar hydrolytic behaviour, as would be expected by their high structural similarity. Cluster analysis, based on functional criteria, seemed to be able to resolve the 19 kDa acidic xylanase from T. reesei from the two 20 kDa xylanases. The 29 kDa xylanase from T. harzianum was the most distinct enzyme and was the best performer in the hydrolysis of four out of the five substrates. The deacetylation of xylan seemed to be able to increase the hydrolysis yields obtained with the first three xylanases
9 citations
Patent•
10 May 1995TL;DR: In this paper, the EG III cellulase enzyme from Trichoderma longibrachiatum and the amino acid sequence of the secreted (mature) and non-secreted (preprotein) forms of the enzyme were presented.
Abstract: The present invention is directed to purified EG III cellulase enzyme isolated from Trichoderma longibrachiatum and the amino acid sequence of the secreted (mature) and non-secreted (preprotein) forms. The present invention is further directed to the DNA fragment and sequence that encodes the EG III cellulase enzyme. Also disclosed are methods for isolating either purified or highly enriched EG III cellulase obtained from Trichoderma spp. or genetically modified strains of Trichoderma spp.
8 citations
Patent•
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24 Oct 1995
TL;DR: The cellulase type enzyme, which is produced by a Trichoderma longibrachiatum and has a >= 3,600 U/ml carboxymethylcellulose activity, is added to the wood pulp in an amount of 0.01-0.2 wt. as discussed by the authors.
Abstract: PURPOSE: To provide a production method of paper wherein the content of fillers in the interior of paper can be increased without reducing the internal bond strength of the interior of paper. CONSTITUTION: The production method of paper containing fillers comprises a step for adding a wood pulp and cellulase type enzyme to a pulper. The cellulase type enzyme, which is produced by a Trichoderma longibrachiatum and has a >=3,600 U/ml carboxymethylcellulose activity, is added to the wood pulp in an amount of 0.01-0.2 wt.% on the basis of the weight of the wood pulp.
2 citations