A. Nasim

TL;DR: Nine radiation-sensitive mutants of S. pombe showing a variety of phenotypic characteristics were analysed for their ability to excise pyrimidine dimers after ultraviolet irradiation, showing that all the mutants tested, like wild type cells, were able to remove a high percentage of pyrimine dimers during post-irradiation incubation.

TL;DR: Observations indicate that the presently available radiation sensitive mutants represent defects in only two major pathways for repair of radiation damage in the wild type S. pombe.

TL;DR: Cells of wild-type Schizosacharomyces pombe exposed to UV radiation in either G1 or G2 phase show enhanced inactivation of colony-forming ability if plated in the presence of caffeine, and the recombinational process therefore may be the major pathway responsible for UV-induced mutation.

TL;DR: The results of both sets of experiments suggested that caffeine interferes with a recombinational repair occurring in cells in S or G2 phase of Saccharomyces cerevisiae.

TL;DR: A simplified procedure for studying pyrimidine dimer induction and excision in yeast DNA has been developed using the use of [14C] uracil as a labelled precursor of both RNA and DNA, followed by efficient removal of RNA.