Calcium ion and muscle contraction.

The value of the adenylate energy charge, [(adenosine triphosphate) + ½ (adenosine diphosphate)]/[(adenosine triphosphate) + (adenosine diphosphate) + (adenosine monophosphate)], in Escherichia coli cells during growth is about 0.8. During the stationary phase after cessation of growth, or during starvation in carbon-limited cultures, the energy charge declines slowly to a value of about 0.5, and then falls more rapidly. During the slow decline in energy charge, all the cells are capable of forming colonies, but a rapid fall in viability coincides with the steep drop in energy charge. These results suggest that growth can occur only at energy charge values above about 0.8, that viability is maintained at values between 0.8 and 0.5, and that cells die at values below 0.5. Tabulation of adenylate concentrations previously reported for various organisms and tissues supports the prediction, based on enzyme kinetic observations in vitro, that the energy charge is stabilized near 0.85 in intact metabolizing cells of a wide variety of types.

Adenylate Energy Charge in Escherichia coli During Growth and Starvation

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Regulation of glucose uptake by muscle. 8. Effects of fatty acids, ketone bodies and pyruvate, and of alloxan-diabetes and starvation, on the uptake and metabolic fate of glucose in rat heart and diaphragm muscles.

Effect of pH on the Kinetics of Frog Muscle Phosphofructokinase

The activity patterns of many sports (e.g. badminton, basketball, soccer and squash) are intermittent in nature, consisting of repeated bouts of brief (≤6-second) maximal/near-maximal work interspersed with relatively short (≤60-second) moderate/low-intensity recovery periods. Although this is a general description of the complex activity patterns experienced in such events, it currently provides the best means of directly assessing the physiological response to this type of exercise. During a single short (5- to 6-second) sprint, adenosine triphosphate (ATP) is resynthesised predominantly from anaerobic sources (phosphocreatine [PCr] degradation and glycolysis), with a small (<10%) contribution from aerobic metabolism. During recovery, oxygen uptake (V-dotO2) remains elevated to restore homeostasis via processes such as the replenishment of tissue oxygen stores, the resynthesis of PCr, the metabolism of lactate, and the removal of accumulated intracellular inorganic phosphate (Pi). If recovery periods are relatively short, V-dotO2 remains elevated prior to subsequent sprints and the aerobic contribution to ATP resynthesis increases. However, if the duration of the recovery periods is insufficient to restore the metabolic environment to resting conditions, performance during successive work bouts may be compromised. Although the precise mechanisms of fatigue during multiple sprint work are difficult to elucidate, evidence points to a lack of available PCr and an accumulation of intracellular Pi as the most likely causes. Moreover, the fact that both PCr resynthesis and the removal of accumulated intracellular Pi are oxygen-dependent processes has led several authors to propose a link between aerobic fitness and fatigue during multiple sprint work. However, whilst the theoretical basis for such a relationship is compelling, corroborative research is far from substantive. Despite years of investigation, limitations in analytical techniques combined with methodological differences between studies have left many issues regarding the physiological response to multiple sprint work unresolved. As such, multiple sprint work provides a rich area for future applied sports science research.

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A. Parmeggiani

Papers

Regulation of phosphofructokinase activity by citrate in normal and diabetic muscle.

Effect of adenine nucleotides and inorganic phosphate on muscle phosphorylase activity.