抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

The term photonic crystals appears because of the analogy between electron waves in crystals and the light waves in artificial periodic dielectric structures. During the recent years the investigation of one-, two-and three-dimensional periodic structures has attracted a widespread attention of the world optics community because of great potentiality of such structures in advanced applied optical fields. The interest in periodic structures has been stimulated by the fast development of semiconductor technology that now allows the fabrication of artificial structures, whose period is comparable with the wavelength of light in the visible and infrared ranges.

http://ieeexplore.ieee.org/iel5/6354/16969/00781867.pdf

Photonic crystals

Sensitive optical biosensors for unlabeled targets: a review.

Microfabrication of graphene devices used in many experimental studies currently relies on the fact that graphene crystallites can be visualized using optical microscopy if prepared on top of Si wafers with a certain thickness of SiO2. The authors study graphene’s visibility and show that it depends strongly on both thickness of SiO2 and light wavelength. They have found that by using monochromatic illumination, graphene can be isolated for any SiO2 thickness, albeit 300nm (the current standard) and, especially, ≈100nm are most suitable for its visual detection. By using a Fresnel-law-based model, they quantitatively describe the experimental data.

/pdf/making-graphene-visible-4fjbh75lug.pdf

Making graphene visible

We present a novel waveguide geometry for enhancing and confining light in a nanometer-wide low-index material. Light enhancement and confinement is caused by large discontinuity of the electric field at highindex-contrast interfaces. We show that by use of such a structure the field can be confined in a 50-nm-wide low-index region with a normalized intensity of 20 mm 22 . This intensity is approximately 20 times higher than what can be achieved in SiO2 with conventional rectangular waveguides. © 2004 Optical Society of America OCIS codes: 030.4070, 130.0130, 130.2790, 230.7370, 230.7380, 230.7390, 230.7400. Recent results in integrated optics have shown the ability to guide, bend, split, and f ilter light on chips by use of optical devices based on high-index-contrast waveguides. 1–5 In all these devices the guiding mechanism is based on total internal ref lection (TIR) in a highindex material (core) surrounded by a low-indexmaterial (cladding); the TIR mechanism can strongly confine light in the high-index material. In recent years a number of structures have been proposed to guide or enhance light in low-index materials, 6–1 1 relying on external ref lections provided by interference effects. Unlike TIR, the external ref lection cannot be perfectly unity; therefore the modes in these structures are inherently leaky modes. In addition, since interference is involved, these structures are strongly wavelength dependent. Here we show that the optical field can be enhanced and conf ined in the low-index material even when light is guided by TIR. For a high-index-contrast interface, Maxwell’s equations state that, to satisfy the continuity of the normal component of electric f lux density D, the corresponding electric field (E-field) must undergo a large discontinuity with much higher amplitude in the low-index side. We show that this discontinuity can be used to strongly enhance and confine light in a nanometer-wide region of low-index material. The proposed structure presents an eigenmode, and it is compatible with highly integrated photonics technology. The principle of operation of the novel structure can be illustrated by analysis of the slab-based structure shown in Fig. 1(a), where a low-index slot is embedded between two high-index slabs (shaded regions). The novel structure is hereafter referred to as a slot waveguide. The slot waveguide eigenmode can be seen as being formed by the interaction between the fundamental eigenmodes of the individual slab waveguides. Rigorously, the analytical solution for the transverse E-field profile Ex of the fundamental TM eigenmode of the slab-based slot waveguide is

/pdf/guiding-and-confining-light-in-void-nanostructure-2oa0yk6hqm.pdf

Guiding and confining light in void nanostructure.

A system and method for performing rapid, automated and high peak capacity separations of complex protein mixtures through the combination of fluidic and electrical elements on an integrated circuit, utilizing planar thin-film micromachining for both fluidic and electrical components.

Integrated planar microfluidic bioanalytical systems

A new waveguide design for an optofluidic chip is presented. It mitigates multi-mode behavior in solid and liquid-core waveguides by increasing fundamental mode coupling to 82% and 95%, respectively. Additionally, we demonstrate a six-fold improvement in lateral confinement of optically guided dielectric microparticles and double the detection efficiency of fluorescent particles.

Multi-mode mitigation in an optofluidic chip for particle manipulation and sensing

Rationale We describe the miniaturization of a linear-type ion trap mass spectrometer for possible applications in portable chemical analysis. This work demonstrates the potential and the advantages of using lithographically patterned electrode plates in realizing an ion trap with dimension y0 less than 1 mm. The focus of this work was to demonstrate the viability and flexibility of the patterned electrode approach to trap miniaturization, and also to discover potential obstacles to its use. Methods Planar, low-capacitance ceramic substrates were patterned with metal electrodes using photolithography. Plates that were originally used in a linear trap with a half-spacing (y0 ) of 2.19 mm were positioned much closer together such that y0 = 0.95 mm. A capacitive voltage divider provided different radiofrequency (RF) amplitudes to each of 10 electrode elements (5 on each side of the ejection slit), and the capacitor values were adjusted to provide the correct electric field at this closer spacing. The length of the trapping region, 45 mm, is unchanged from the previous device. Results Electron ionization mass spectra of toluene and dichloromethane demonstrate instrument performance, with better than unit mass resolution for the molecular ion and fragment ion peaks of toluene. Compared with the larger plate spacing, the signal is reduced, corresponding to the reduced trapping capacity of the smaller device. However, the mass resolution of the larger device is retained. Conclusions Lithographically patterned substrates are a viable pathway to fabricating highly miniaturized ion traps for mass spectrometry. These results also demonstrate the possibility of significant reduction of the ion trap volume without physical modification of the electrodes. These experiments show promise for further miniaturization using assemblies of patterned ceramic plates. Copyright © 2014 John Wiley & Sons, Ltd.

Miniaturization of a planar‐electrode linear ion trap mass spectrometer

Planar Si/InGaAs wafer fused p-i-n photodetectors were fabricated and measured. They show high internal quantum efficiency, high speed, record low dark current, and no evidence of charge trapping, recombination centers, or a bandgap discontinuity at the heterointerface.

20 ghz high performance planar si/ingaas p-i-n photodetector

Over the past decade, optofluidics has established itself as a new and dynamic research field for exciting developments at the interface of photonics, microfluidics, and the life sciences. The strong desire for developing miniaturized bioanalytic devices and instruments, in particular, has led to novel and powerful approaches to integrating optical elements and biological fluids on the same chip-scale system. Here, we review the state-of-the-art in optofluidic research with emphasis on applications in bioanalysis and a focus on waveguide-based approaches that represent the most advanced level of integration between optics and fluidics. We discuss recent work in photonically reconfigurable devices and various application areas. We show how optofluidic approaches have been pushing the performance limits in bioanalysis, e.g. in terms of sensitivity and portability, satisfying many of the key requirements for point-of-care devices. This illustrates how the requirements for bianalysis instruments are increasingly being met by the symbiotic integration of novel photonic capabilities in a miniaturized system.

/pdf/optofluidic-bioanalysis-fundamentals-and-applications-wyio9zt0i9.pdf

Aaron R. Hawkins

Papers

Integrated planar microfluidic bioanalytical systems

Multi-mode mitigation in an optofluidic chip for particle manipulation and sensing

Miniaturization of a planar‐electrode linear ion trap mass spectrometer

20 ghz high performance planar si/ingaas p-i-n photodetector

Optofluidic bioanalysis: fundamentals and applications.