Wound healing is a complex process in tissue regeneration through which the body responds to the dissipated cells as a result of any kind of severe injury. Diabetic and non‐healing wounds are considered an unmet clinical need. Currently, different strategic approaches are widely used in the treatment of acute and chronic wounds which include, but are not limited to, tissue transplantation, cell therapy and wound dressings, and the use of an instrument. A large number of literatures have been published on this topic; however, the most effective clinical treatment remains a challenge. The wound dressing involves the use of a scaffold, usually using biomaterials for the delivery of medication, autologous stem cells, or growth factors from the blood. Antibacterial and anti‐inflammatory drugs are also used to stop the infection as well as accelerate wound healing. With an increase in the ageing population leading to diabetes and associated cutaneous wounds, there is a great need to improve the current treatment strategies. This research critically reviews the current advancement in the therapeutic and clinical approaches for wound healing and tissue regeneration. The results of recent clinical trials suggest that the use of modern dressings and skin substitutes is the easiest, most accessible, and most cost‐effective way to treat chronic wounds with advances in materials science such as graphene as 3D scaffold and biomolecules hold significant promise. The annual market value for successful wound treatment exceeds over $50 billion US dollars, and this will encourage industries as well as academics to investigate the application of emerging smart materials for modern dressings and skin substitutes for wound therapy.

https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/iwj.13786

Emerging treatment strategies in wound care

Electronic products are becoming an essential part of our daily life. There is a huge demand to produce small and portable but powerful electronic products. Carbon nanotubes (CNTs) have excellent electrical, mechanical and thermal properties which can be exploited to build next-generation electronics. This paper reviews different types and properties of CNTs and also presents the CNT-based electronics along with their advantage over the conventionally used products. CNT usage in electronics, such as biosensing, energy and data storage devices, is discussed. CNT-based field emission devices, which showed outstanding results are also discussed. The current challenges of CNT-based electronics and the future of CNT in electronics applications are mentioned.Graphical Abstract 

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A Critical Review of the Role of Carbon Nanotubes in the Progress of Next-Generation Electronic Applications

A wound is defined as damage to the integrity of biological tissue, including skin, mucous membranes, and organ tissues. The treatment of these injuries is an important challenge for medical researchers. Various materials have been used for wound healing and dressing applications among which carbon nanomaterials have attracted significant attention due to their remarkable properties. In the present review, the latest studies on the application of carbon nanomaterials including graphene oxide (GO), reduced graphene oxide (rGO), carbon dots (CDs), carbon quantum dots (CQDs), carbon nanotubes (CNTs), carbon nanofibers (CNFs), and nanodiamonds (NDs) in wound dressing applications are evaluated. Also, a variety of carbon-based nanocomposites with advantages such as biocompatibility, hemocompatibility, reduced wound healing time, antibacterial properties, cell-adhesion, enhanced mechanical properties, and enhanced permeability to oxygen has been reported for the treatment of various wounds.

A comprehensive review on the applications of carbon-based nanostructures in wound healing: from antibacterial aspects to cell growth stimulation.

Methylene blue is a toxic dye that is extensively used as a colorant in textile industries. Industrial effluent containing methylene blue, when drained into water bodies without proper treatment, poses a serious threat to aquatic and human lives. In order to protect the biocycle, various methods have been established to detect and remove hazardous dyes from aqueous systems. Electrochemical methods are preferred, owing to their characteristic features of simplicity, portability, potential selectivity, cost effectiveness, and rapid responsiveness. Based on these considerations, an electrochemical sensor consisting of amino-group-functionalized, multi-walled carbon nanotubes (NH2-fMWCNTs) immobilized on a glassy carbon electrode (GCE) was developed for the sensitive detection of methylene blue in aqueous solutions. The performance of the designed sensor was analyzed by electrochemical impedance spectroscopy, cyclic voltammetry, and square wave voltammetry. The developed sensing tool demonstrated promising features of sensitivity, selectivity, stability, fast responsiveness, and the ability to work with a very small volume of the analyte, i.e., in microliters, for analysis. Amino groups rich in electrons provide a negative charge to multi-walled carbon nanotubes, which significantly enhances the electrocatalytic activity of NH2-fMWCNTs for cationic dyes such as methylene blue. Using the designed sensing platform, a linear calibration plot with a limit of detection of 0.21 nM was obtained for methylene blue under optimized conditions. The designed sensor was also employed to monitor the extent and kinetics of the degradation of methylene blue. Titania nanoparticles were used for photocatalytic degradation, and the kinetics of degradation was monitored by both UV-Visible spectroscopic and electrochemical methods. The results revealed more than 95% removal of methylene blue in a time span of just 30 min.

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A Novel Electrochemical Sensing Platform for the Sensitive Detection and Degradation Monitoring of Methylene Blue

Microbial diseases have been declared one of the main threats to humanity, which is why, in recent years, great interest has been generated in the development of nanocomposites with antimicrobial capacity. The present work studied two magnetic nanocomposites based on graphene oxide (GO) and multiwall carbon nanotubes (MWCNTs). The synthesis of these magnetic nanocomposites consisted of three phases: first, the synthesis of iron magnetic nanoparticles (MNPs), second, the adsorption of the photosensitizer menthol-Zinc phthalocyanine (ZnMintPc) into MWCNTs and GO, and the third phase, encapsulation in poly (N-vinylcaprolactam-co-poly(ethylene glycol diacrylate)) poly (VCL-co-PEGDA) polymer VCL/PEGDA a biocompatible hydrogel, to obtain the magnetic nanocomposites VCL/PEGDA-MNPs-MWCNTs-ZnMintPc and VCL/PEGDA-MNPs-GO-ZnMintPc. In vitro studies were carried out using Escherichia coli and Staphylococcus aureus bacteria and the Candida albicans yeast based on the Photodynamic/Photothermal (PTT/PDT) effect. This research describes the nanocomposites’ optical, morphological, magnetic, and photophysical characteristics and their application as antimicrobial agents. The antimicrobial effect of magnetics nanocomposites was evaluated based on the PDT/PTT effect. For this purpose, doses of 65 mW·cm−2 with 630 nm light were used. The VCL/PEGDA-MNPs-GO-ZnMintPc nanocomposite eliminated E. coli and S. aureus colonies, while the VCL/PEGDA-MNPs-MWCNTs-ZnMintPc nanocomposite was able to kill the three types of microorganisms. Consequently, the latter is considered a broad-spectrum antimicrobial agent in PDT and PTT.

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Broad-Spectrum Antimicrobial ZnMintPc Encapsulated in Magnetic-Nanocomposites with Graphene Oxide/MWCNTs Based on Bimodal Action of Photodynamic and Photothermal Effects

Biocompatible nanomaterials have attracted enormous interest for biomedical applications. Carbonaceous materials, including carbon nanotubes (CNTs), have been widely explored in wound healing and other applications because of their superior physicochemical and potential biomedical properties to the nanoscale level. CNTs-based hydrogels are widely used for wound-healing and antibacterial applications. CNTs-based materials exhibited improved antimicrobial, antibacterial, adhesive, antioxidants, and mechanical properties, which are beneficial for the wound-healing process. This review concisely discussed the preparation of CNTs-based hydrogels and their antibacterial and wound-healing applications. The conductive potential of CNTs and their derivatives is discussed. It has been observed that the conductivity of CNTs is profoundly affected by their structure, temperature, and functionalization. CNTs properties can be easily modified by surface functionalization. CNTs-based composite hydrogels demonstrated superior antibacterial potential to corresponding pure polymer hydrogels. The accelerated wound healing was observed with CNTs-based hydrogels.

Carbon Nanotubes-Based Hydrogels for Bacterial Eradiation and Wound-Healing Applications

Cellulose is the most venerable and essential natural polymer on the planet and is drawing greater attention in the form of nanocellulose, considered an innovative and influential material in the biomedical field. Because of its exceptional physicochemical characteristics, biodegradability, biocompatibility, and high mechanical strength, nanocellulose attracts considerable scientific attention. Plants, algae, and microorganisms are some of the familiar sources of nanocellulose and are usually grouped as cellulose nanocrystal (CNC), cellulose nanofibril (CNF), and bacterial nanocellulose (BNC). The current review briefly highlights nanocellulose classification and its attractive properties. Further functionalization or chemical modifications enhance the effectiveness and biodegradability of nanocellulose. Nanocellulose-based composites, printing methods, and their potential applications in the biomedical field have also been introduced herein. Finally, the study is summarized with future prospects and challenges associated with the nanocellulose-based materials to promote studies resolving the current issues related to nanocellulose for tissue engineering applications.

/pdf/a-review-of-properties-of-nanocellulose-its-synthesis-and-kfibjzob.pdf

A Review of Properties of Nanocellulose, Its Synthesis, and Potential in Biomedical Applications

Electrically stimulated 3D bioprinting of gelatin-polypyrrole hydrogel with dynamic semi-IPN network induces osteogenesis via collective signaling and immunopolarization.

The conversion of liquid resin into solid structures upon exposure to light of a specific wavelength is known as photopolymerization or photo-curable 3D printing. In recent years, photopolymerization-based 3D printing has gained enormous attention in tissue engineering for constructing highly complex and precise tissue structures. Due to the economic and environmental benefits of the biopolymers employed, photo-curable 3D printing is considered highly accurate and an alternative method for replacing damaged tissues. However, the lack of suitable bio-based photopolymers, their characterization, effective crosslinking strategies, and optimal printing conditions are hindering the extensive application and commercialization of those materials in the global market. This review highlights the present status of various photopolymers, their synthesis techniques, and optimization parameters for biomedical applications. Moreover, we also discussed a glimpse of various photopolymerization techniques that are currently employed for 3D printing. Furthurmore, we also reviewed the influence of various naturally-derived nanomaterial reinforced polymerization and their influence on printability and shape fidelity. Finally, the ultimate use of those photopolymerized hydrogel scaffolds in tissue engineering is also discussed. Taken together, we believe that photopolymerized 3D printing has a great future, whereas conventional 3D printing requires considerable sophistication, and this review will provide the readers with a comprehensive approach to the development of light-mediated 3D printing for tissue engineering applications. This article is protected by copyright. All rights reserved.

Recent Advances in 3D Printing of Photocurable Polymers: Types, Mechanism, and Tissue Engineering Application.

Biomimetic soft hydrogels used in bone tissue engineering frequently produce unsatisfactory outcomes. Here, it is investigated how human bone‐marrow‐derived mesenchymal stem cells (hBMSCs) differentiated into early osteoblasts on remarkably soft 3D hydrogel (70 ± 0.00049 Pa). Specifically, hBMSCs seeded onto cellulose nanocrystals incorporated methacrylate gelatin hydrogels are subjected to pulsatile pressure stimulation (PPS) of 5–20 kPa for 7 days. The PPS stimulates cellular processes such as mechanotransduction, cytoskeletal distribution, prohibition of oxidative stress, calcium homeostasis, osteogenic marker gene expression, and osteo‐specific cytokine secretions in hBMSCs on soft substrates. The involvement of Piezo 1 is the main ion channel involved in mechanotransduction. Additionally, RNA‐sequencing results reveal differential gene expression concerning osteogenic differentiation, bone mineralization, ion channel activity, and focal adhesion. These findings suggest a practical and highly scalable method for promoting stem cell commitment to osteogenesis on soft matrices for clinical reconstruction.

Transcriptomic Changes toward Osteogenic Differentiation of Mesenchymal Stem Cells on 3D‐Printed GelMA/CNC Hydrogel under Pulsatile Pressure Environment

Aayushi Randhawa

Papers

Carbon Nanotubes-Based Hydrogels for Bacterial Eradiation and Wound-Healing Applications

A Review of Properties of Nanocellulose, Its Synthesis, and Potential in Biomedical Applications

Electrically stimulated 3D bioprinting of gelatin-polypyrrole hydrogel with dynamic semi-IPN network induces osteogenesis via collective signaling and immunopolarization.

Recent Advances in 3D Printing of Photocurable Polymers: Types, Mechanism, and Tissue Engineering Application.

Transcriptomic Changes toward Osteogenic Differentiation of Mesenchymal Stem Cells on 3D‐Printed GelMA/CNC Hydrogel under Pulsatile Pressure Environment