Oral microbiology with basic microbiology and immunology

Influence of black tea on Streptococcus mutans and Lactobacillus levels in saliva in a Saudi cohort.

/pdf/a-biochemical-study-of-streptococci-with-special-emphasis-on-udbw920m7x.pdf

A biochemical study of streptococci, with special emphasis on the determination of their chemical composition ... by Frances Krasnow ...

Background: The green tea have antimicrobial activity against many types of bacteria It is considered a natural substance with few side effect.
        Aim of the Study: An in vitro study was carried out to investigate the ability of green tea extract and nicotine to inhibit growth, biofilm formation by salivary Mutans streptococci.
Methods: This study included a convenient sample of 40 healthy Iraqi volunteers aged 18–23 years old from College of Dentistry / University of Baghdad. Green tea and nicotine aqueous extract were prepared in different concentration to use in agar diffusion method to detect the activity of extract, and ELISA reader in multi titer plate was used to determine the ability of salivary mutans Streptococci to form biofilm in the presence and absence of the extracts.to measure the biofilm inhibition rate.
        Results: Mutans Streptococci were sensitive to green tea and nicotine in different concentrations     the diameters of the inhibition zone were effective in a dose dependent manner significantly. There was a significant difference between the concentrations of each extracts, antibacterial activity was in a dose dependent manner for the extracts. The minimum bactericidal concentration of green tea was (280 mg/ml) and minimum bactericidal concentration of nicotine was (45mg/ml). The study found that biofilm formation by Mutans Streptococci was markedly decreased in the presence of 1/2 minimum bactericidal concentration of both green tea and nicotine with mean of O.D 590 nm = 0.54 in comparison with green tea extracts and nicotine alone O.D 590 nm = 0.15, 0.68 respectively.
Conclusions green tea and nicotine extracts in different concentration effectively reduced the biofilm formation of salivary Mutans streptococci. While the presence of nicotine has negatively impacted on the ability of green tea extracts in the inhibition of biofilm formation by Mutans Streptococci in vitro.

https://doi.org/10.32007/jfacmedbagdad.2024

Anti-microbial activity of Green Tea Extracts and Nicotine on the Growth, Biofilm Formation of Salivary Mutans Streptococci (In-vitro study)

Oral diseases, while largely preventable, pose a major health burden for many countries and affect people throughout their lifetime, causing pain, discomfort and disfigurement. Mouth washing agents are an effective vehicle for antimicrobial compounds in reducing oral pathogens, managing dental plaque and maintaining proper oral health. Numerous plant extracts have been successfully incorporated into oral care products due to their antimicrobial properties, availability, and affordability. Tea plants and extracts are one of the most important natural compounds used in dental care products. The problem with tea extracts is their bitter taste, which could compromise their usage by people. So, it needs a kind of supplementation to be palatable, especially for the pediatric and geriatric populations. Hence a nonnutritive sweetener was added to tea extracts to suppress their bitter taste. This study aimed to determine the effect of nonnutritive sweeteners on the antifungal activity of black and green tea aqueous extracts against salivary C. albicans. The results showed that adding 1% of stevia and sucralose to the black and green tea aqueous extracts did not affect the antifungal activity against salivary C. 

The effect of nonnutritive sweeteners on the antifungal activity of black and green tea aqueous extracts against salivary Candida albicans an (in-vitro study)

Abbas S. Al-Mizrakchi

Papers

Black and green tea antimicrobial effect on Mutans streptococci and Lactobacilli