Adrian P. Wiegmans

TL;DR: It is shown that concentration of cell culture conditioned media using ultrafiltration devices results in increased vesicle isolation when compared to traditional ultracentrifugation protocols, and it is demonstrated that size exclusion isolation is comparable to density gradient purification of exosomes.

TL;DR: It is proposed that promising combinations of DNA-damaging anticancer treatments with DDR-pathway inhibition would be further enhanced by activating downstream apoptotic pathways, ensuring that actual cell death is the preferred outcome of cancer treatment instead of other responses, including reversible cell cycle arrest, autophagy or senescence.

TL;DR: The potential treatment efficacy and future clinical applications of RAD51 inhibitors as single agents and in combination with other therapies are scrutinized and the best therapeutic options are considered.

TL;DR: Analysis of immunohistochemistry in an independent cohort of 235 were sporadic breast tumours suggested that RAD51 expression is increased during breast cancer progression and metastasis and an oncogenic role for RAD51 when deregulated and subsequent knockdown repressed cancer cell migration in vitro and reduced primary tumor growth in a syngeneic mouse model in vivo.

TL;DR: The combination of Erk5 and Hsp90 inhibitors was effective in vitro and in vivo against TNBC leading to upregulation of pro-apoptotic effectors, contributing to proteomic profiling and improving understanding of TNBC heterogeneity to provide therapeutic opportunities for this disease.