Richard J. Lobb

TL;DR: It is shown that concentration of cell culture conditioned media using ultrafiltration devices results in increased vesicle isolation when compared to traditional ultracentrifugation protocols, and it is demonstrated that size exclusion isolation is comparable to density gradient purification of exosomes.

TL;DR: This Review highlights the progress the cancer sEV field has made in the areas of biomarker discovery and validation as well as sEV-based therapeutics, highlights the challenges the authors are facing and identifies gaps in knowledge, which currently prevent the full potential of sEVs in cancer diagnostic and therapy.

TL;DR: It is determined that breast cancer exosomes directly suppressed T-cell proliferation and inhibited NK cell cytotoxicity, and hence likely suppressed the anticancer immune response in premetastatic organs.

TL;DR: The recent findings that implicate this non-canonical signalling within the tumour as a critical driver of metastatic disease progression are summarized, and how understanding the molecular mechanisms involved in exosome-mediated metastasis is of great value for the development of new therapeutic strategies to prevent cancer progression is discussed.

TL;DR: It is demonstrated that breast cancer-derived exosomes are capable of inducing IL-6 secretion and a pro-survival phenotype in macrophages, partially via gp130/STAT3 signaling.