Detection, evaluation, and treatment of high blood cholesterol in adults (Adult Treatment Panel III)

In 1901 a physician, Archibald Garrod, observed a patient with black urine. He used this simple observation to demonstrate that a single mutant gene can produce a discrete block in a biochemical pathway, which he called an “inborn error of metabolism”. Garrod’s brilliant insight anticipated by 40 years the one gene-one enzyme concept of Beadle and Tatum. In similar fashion the chemist Linus Pauling and the biochemist Vernon Ingram, through study of patients with sickle cell anemia, showed that mutant genes alter the amino acid sequences of proteins. Clearly, many fundamental advances in biology were spawned by perceptive studies of human genetic diseases (1). We began our work in 1972 in an attempt to understand a human genetic disease, familial hypercholesterolemia or FH. In these patients the concentration of cholesterol in blood is elevated many fold above normal and heart attacks occur early in life. We postulated that this dominantly inherited disease results from a failure of end-product repression of cholesterol synthesis. The possibility fascinated us because genetic defects in feedback regulation had not been observed previously in humans or animals, and we hoped that study of this disease might throw light on fundamental regulatory mechanisms. Our approach was to apply the techniques of cell culture to unravel the postulated regulatory defect in FH. These studies led to the discovery of a cell surface receptor for a plasma cholesterol transport protein called low density lipoprotein (LDL) and to the elucidation of the mechanism by which this receptor mediates feedback control of cholesterol synthesis (2,3). FH was shown to be caused by inherited defects in the gene encoding the LDL receptor, which disrupt the normal control of cholesterol metabolism. Study of the LDL receptor in turn led to the understanding of receptor-mediated endocytosis, a genera! process by which cells communicate with each other through internalization of regulatory and nutritional molecules (4). Receptor-mediated endocytosis differs from previously described biochemical pathways because it depends upon the continuous and highly controlled movement of membraneembedded proteins from one cell organelle to another in a process termed

https://science.sciencemag.org/content/sci/232/4746/34.full.pdf

A receptor-mediated pathway for cholesterol homeostasis.

Flavonoids are nearly ubiquitous in plants and are recognized as the pigments responsible for the colors of leaves, especially in autumn. They are rich in seeds, citrus fruits, olive oil, tea, and red wine. They are low molecular weight compounds composed of a three-ring structure with various substitutions. This basic structure is shared by tocopherols (vitamin E). Flavonoids can be subdivided according to the presence of an oxy group at position 4, a double bond between carbon atoms 2 and 3, or a hydroxyl group in position 3 of the C (middle) ring. These characteristics appear to also be required for best activity, especially antioxidant and antiproliferative, in the systems studied. The particular hydroxylation pattern of the B ring of the flavonoles increases their activities, especially in inhibition of mast cell secretion. Certain plants and spices containing flavonoids have been used for thousands of years in traditional Eastern medicine. In spite of the voluminous literature available, however, Western medicine has not yet used flavonoids therapeutically, even though their safety record is exceptional. Suggestions are made where such possibilities may be worth pursuing.

The Effects of Plant Flavonoids on Mammalian Cells:Implications for Inflammation, Heart Disease, and Cancer

A B S T R AC T BACKGROUND The order and magnitude of pathologic processes in Alzheimer’s disease are not well understood, partly because the disease develops over many years. Autosomal dominant Alzheimer’s disease has a predictable age at onset and provides an opportunity to determine the sequence and magnitude of pathologic changes that culminate in symptomatic disease. METHODS In this prospective, longitudinal study, we analyzed data from 128 participants who underwent baseline clinical and cognitive assessments, brain imaging, and cerebrospinal fluid (CSF) and blood tests. We used the participant’s age at baseline assessment and the parent’s age at the onset of symptoms of Alzheimer’s disease to calculate the estimated years from expected symptom onset (age of the participant minus parent’s age at symptom onset). We conducted cross-sectional analyses of baseline data in relation to estimated years from expected symptom onset in order to determine the relative order and magnitude of pathophysiological changes. RESULTS Concentrations of amyloid-beta (Aβ)42 in the CSF appeared to decline 25 years before expected symptom onset. Aβ deposition, as measured by positron-emission tomography with the use of Pittsburgh compound B, was detected 15 years before expected symptom onset. Increased concentrations of tau protein in the CSF and an increase in brain atrophy were detected 15 years before expected symptom onset. Cerebral hypometabolism and impaired episodic memory were observed 10 years before expected symptom onset. Global cognitive impairment, as measured by the Mini–Mental State Examination and the Clinical Dementia Rating scale, was detected 5 years before expected symptom onset, and patients met diagnostic criteria for dementia at an average of 3 years after expected symptom onset. CONCLUSIONS We found that autosomal dominant Alzheimer’s disease was associated with a series of pathophysiological changes over decades in CSF biochemical markers of Alzheimer’s disease, brain amyloid deposition, and brain metabolism as well as progressive cognitive impairment. Our results require confirmation with the use of longitudinal data and may not apply to patients with sporadic Alzheimer’s disease. (Funded by the National Institute on Aging and others; DIAN ClinicalTrials.gov number, NCT00869817.)

/pdf/clinical-and-biomarker-changes-in-dominantly-inherited-66jpsco78g.pdf

Clinical and Biomarker Changes in Dominantly Inherited Alzheimer’s Disease

Natural killer T (NKT) lymphocytes express an invariant T cell antigen receptor (TCR) encoded by the Valpha14 and Jalpha281 gene segments. A glycosylceramide-containing alpha-anomeric sugar with a longer fatty acyl chain (C26) and sphingosine base (C18) was identified as a ligand for this TCR. Glycosylceramide-mediated proliferative responses of Valpha14 NKT cells were abrogated by treatment with chloroquine-concanamycin A or by monoclonal antibodies against CD1d/Vbeta8, CD40/CD40L, or B7/CTLA-4/CD28, but not by interference with the function of a transporter-associated protein. Thus, this lymphocyte shares distinct recognition systems with either T or NK cells.

http://science.sciencemag.org/content/sci/278/5343/1626.full.pdf

CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides.

In a previous study (A. ENDO, et al., J. Antibiotics 38: 599 approximately 604, 1985), 2-alkyl glutarate and its derivatives isolated from cultures of Gongronella butleri were shown to inhibit animal acetyl-CoA carboxylase. In the present communication, the inhibition of liver acetyl-CoA carboxylase was investigated with several 2-alkyl glutarate and 2-alkyl succinate analogs. Their inhibitory potency increased with the chain length of the alkyl moiety, and 2-tetradecanylglutarate was most potent among the inhibitors tested. Kinetic analysis indicated that inhibition by 2-tetradecanylglutarate was non-competitive with respect to the substrates, ATP, HCO3- and acetyl-CoA, and competitive with respect to the allosteric regulatory citrate, giving a Ki value of 40 microM. Sucrose density gradient centrifugation analysis showed that the citrate-induced polymerization of the enzyme was inhibited by 2-tetradecanylglutarate.

Inhibition of liver acetyl-coenzyme-A carboxylase by 2-tetradecanylglutarate.

Unstable amplification of the chromosomal gene for 3-hydroxy-3-methylglutaryl coenzyme A reductase in compactin-resistant CR200 cells

Mutastein, a casein polymer that inhibits insoluble glucan synthesis by Streptococcus sobrinus, was produced when Aspergillus terreus was grown in a medium containing α-casein. In these experiments, it was shown that flavipin (3,4,5-trihydroxy-6-methyl-o-phthalaldehyde) isolated from cultures of A. terreus caused polymerization of α-casein. Like mutastein, the polymerization products were active in inhibiting insoluble glucan synthesis by S. sobrinus.

Inhibition of Glucan Synthesis by Flavipin-crosslinked Casein Polymers.

数種の酵素反応 によ り合成 されるが(図-1),HMG(3hydroxy-3-methylglutary1)一CoAレ ダクターゼが その律 速 酵素 であるこ とは,種 々の生理条 件の変化,薬 物 ・ホ ルモ ンの投与及 び 日内周期 な どでみ られ る活 性 の 変 化 と,コ レス テロール合成 の変化 が完全 に対応 する ことか ら一般的 に支持 され ている1)・2)。 物質代謝系の律速酵素 は一般 に多様 な機構 によ り調 節 されてV・る場合が多V・。HMG-CoAレ ダクターゼ も以 下 に紹介す るよ うに,リ ン酸化一脱 リン酸化修飾,ホ ル モ ンの関与,LDLに よるフ ィー ドバ ック制御,さ らに 多価 フ ィー ドバ ック的制御 といった幾重 にも重 な った機 構 によ り調 節 されてい る。 これ らの調節 は,酵 素 を即座 に活 性化 ・不活 性化す る短期調節 と,酵 素 タ ンパ クの合 成 または分解速度 の調節 とい う,長 ・短2つ の機構 か ら 成 る。 1HMG-CoAレ ダ ク タ ーゼ の\"リ ン酸 化 一脱 リン酸 化\"に よ る調 節

Regulation of Cholesterol Synthesis

PROBLEM TO BE SOLVED: To receive energy information and production information of a production facility, and perform automatic drill-down analysis for CO 2 emission in a factory, a production plant or the like, thereby remarkably reducing man hours to manage CO 2 emission of a congested production facility SOLUTION: The production energy monitoring control system is provided in which a plurality of energy management systems constructed for each facility such as a building-utility facility and the production facility are connected to a common management center via a network for centralized management The management center includes a production CO 2 management system for collecting data such as the energy information, environmental information and operation information from the building-utility facility and the production facility, and for carrying out automatic drill-down analysis for CO 2 management based on the collected data COPYRIGHT: (C)2010,JPO&INPIT

Akira Endo

Papers

Inhibition of liver acetyl-coenzyme-A carboxylase by 2-tetradecanylglutarate.

Unstable amplification of the chromosomal gene for 3-hydroxy-3-methylglutaryl coenzyme A reductase in compactin-resistant CR200 cells

Inhibition of Glucan Synthesis by Flavipin-crosslinked Casein Polymers.

Regulation of Cholesterol Synthesis

Production energy monitoring control system