Detection, evaluation, and treatment of high blood cholesterol in adults (Adult Treatment Panel III)

In 1901 a physician, Archibald Garrod, observed a patient with black urine. He used this simple observation to demonstrate that a single mutant gene can produce a discrete block in a biochemical pathway, which he called an “inborn error of metabolism”. Garrod’s brilliant insight anticipated by 40 years the one gene-one enzyme concept of Beadle and Tatum. In similar fashion the chemist Linus Pauling and the biochemist Vernon Ingram, through study of patients with sickle cell anemia, showed that mutant genes alter the amino acid sequences of proteins. Clearly, many fundamental advances in biology were spawned by perceptive studies of human genetic diseases (1). We began our work in 1972 in an attempt to understand a human genetic disease, familial hypercholesterolemia or FH. In these patients the concentration of cholesterol in blood is elevated many fold above normal and heart attacks occur early in life. We postulated that this dominantly inherited disease results from a failure of end-product repression of cholesterol synthesis. The possibility fascinated us because genetic defects in feedback regulation had not been observed previously in humans or animals, and we hoped that study of this disease might throw light on fundamental regulatory mechanisms. Our approach was to apply the techniques of cell culture to unravel the postulated regulatory defect in FH. These studies led to the discovery of a cell surface receptor for a plasma cholesterol transport protein called low density lipoprotein (LDL) and to the elucidation of the mechanism by which this receptor mediates feedback control of cholesterol synthesis (2,3). FH was shown to be caused by inherited defects in the gene encoding the LDL receptor, which disrupt the normal control of cholesterol metabolism. Study of the LDL receptor in turn led to the understanding of receptor-mediated endocytosis, a genera! process by which cells communicate with each other through internalization of regulatory and nutritional molecules (4). Receptor-mediated endocytosis differs from previously described biochemical pathways because it depends upon the continuous and highly controlled movement of membraneembedded proteins from one cell organelle to another in a process termed

https://science.sciencemag.org/content/sci/232/4746/34.full.pdf

A receptor-mediated pathway for cholesterol homeostasis.

Flavonoids are nearly ubiquitous in plants and are recognized as the pigments responsible for the colors of leaves, especially in autumn. They are rich in seeds, citrus fruits, olive oil, tea, and red wine. They are low molecular weight compounds composed of a three-ring structure with various substitutions. This basic structure is shared by tocopherols (vitamin E). Flavonoids can be subdivided according to the presence of an oxy group at position 4, a double bond between carbon atoms 2 and 3, or a hydroxyl group in position 3 of the C (middle) ring. These characteristics appear to also be required for best activity, especially antioxidant and antiproliferative, in the systems studied. The particular hydroxylation pattern of the B ring of the flavonoles increases their activities, especially in inhibition of mast cell secretion. Certain plants and spices containing flavonoids have been used for thousands of years in traditional Eastern medicine. In spite of the voluminous literature available, however, Western medicine has not yet used flavonoids therapeutically, even though their safety record is exceptional. Suggestions are made where such possibilities may be worth pursuing.

The Effects of Plant Flavonoids on Mammalian Cells:Implications for Inflammation, Heart Disease, and Cancer

A B S T R AC T BACKGROUND The order and magnitude of pathologic processes in Alzheimer’s disease are not well understood, partly because the disease develops over many years. Autosomal dominant Alzheimer’s disease has a predictable age at onset and provides an opportunity to determine the sequence and magnitude of pathologic changes that culminate in symptomatic disease. METHODS In this prospective, longitudinal study, we analyzed data from 128 participants who underwent baseline clinical and cognitive assessments, brain imaging, and cerebrospinal fluid (CSF) and blood tests. We used the participant’s age at baseline assessment and the parent’s age at the onset of symptoms of Alzheimer’s disease to calculate the estimated years from expected symptom onset (age of the participant minus parent’s age at symptom onset). We conducted cross-sectional analyses of baseline data in relation to estimated years from expected symptom onset in order to determine the relative order and magnitude of pathophysiological changes. RESULTS Concentrations of amyloid-beta (Aβ)42 in the CSF appeared to decline 25 years before expected symptom onset. Aβ deposition, as measured by positron-emission tomography with the use of Pittsburgh compound B, was detected 15 years before expected symptom onset. Increased concentrations of tau protein in the CSF and an increase in brain atrophy were detected 15 years before expected symptom onset. Cerebral hypometabolism and impaired episodic memory were observed 10 years before expected symptom onset. Global cognitive impairment, as measured by the Mini–Mental State Examination and the Clinical Dementia Rating scale, was detected 5 years before expected symptom onset, and patients met diagnostic criteria for dementia at an average of 3 years after expected symptom onset. CONCLUSIONS We found that autosomal dominant Alzheimer’s disease was associated with a series of pathophysiological changes over decades in CSF biochemical markers of Alzheimer’s disease, brain amyloid deposition, and brain metabolism as well as progressive cognitive impairment. Our results require confirmation with the use of longitudinal data and may not apply to patients with sporadic Alzheimer’s disease. (Funded by the National Institute on Aging and others; DIAN ClinicalTrials.gov number, NCT00869817.)

/pdf/clinical-and-biomarker-changes-in-dominantly-inherited-66jpsco78g.pdf

Clinical and Biomarker Changes in Dominantly Inherited Alzheimer’s Disease

Natural killer T (NKT) lymphocytes express an invariant T cell antigen receptor (TCR) encoded by the Valpha14 and Jalpha281 gene segments. A glycosylceramide-containing alpha-anomeric sugar with a longer fatty acyl chain (C26) and sphingosine base (C18) was identified as a ligand for this TCR. Glycosylceramide-mediated proliferative responses of Valpha14 NKT cells were abrogated by treatment with chloroquine-concanamycin A or by monoclonal antibodies against CD1d/Vbeta8, CD40/CD40L, or B7/CTLA-4/CD28, but not by interference with the function of a transporter-associated protein. Thus, this lymphocyte shares distinct recognition systems with either T or NK cells.

http://science.sciencemag.org/content/sci/278/5343/1626.full.pdf

CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides.

NEW MATERIAL:The compound of formula I [R1-R3 are H, alkyl, alkali metal, alkaline earth metal, Al, Fe, Zn, Cu, Ni or Co; at least one of R1-R3 is metal; n1-n3 are atomic valence of R1-R3; R4 is H or methyl; excluding the case that R1 is H or methyl and both R2 and R3 are H]. USE:It has cholesterol-lowering activity and is useful as a remedy for hyperlipemia. PREPARATION:The objective compound can be prepared e.g. by reacting the compound of formula II (R5 is H or methyl), e.g. CB3A (R5 is CH3 and R4 is H) or CK3A (both R5 and R4 are CH3) directly with a hydroxide such as NaOH, Ca(OH)2, etc., or a chloride such as CaCl2, or by substituting the alkali metal salt of CB3A or CK3A with a metallic salt such as CaCl2, BaCl2, etc.

Organophosphoric acid compound metal salt and remedy for hyperlipemia containing same

Glutaraldehyde-crosslinked α-casein polymers with molecular masses of 1-5×10(5) were found to inhibit adhesive insoluble glucan formation catalyzed by glucan synthases from Streptococcus sobrinus B13, a cariogenic oral bacterium. Of the three subcomponents of the glucan synthases tested, primer-dependent insoluble glucan synthase was inhibited specifically by the M r~2.7×10(5) polymer at a concentration of 1.4-8.5 μg/ml. The polymer was also active in inhibiting the artificial plaque formation by S. sobrinus B13.

Inhibition of Glucan Synthesis by Casein Polymers Crosslinked by Glutaraldehyde.

A metal salt of an organic phosphate, having good stability and cholesterol-lowering activity, is produced by reacting a known compound ##STR1## wherein R 4 and R 5 are hydrogen or methyl, with a reaction agent such as metal hydroxide.

Metal salt of organic phosphate and an antihyperlipemic agent containing the same

Basic and Developmental Studies of Inhibitors of Cholesterol Biosynthesis

Some tetracycline (TC) antibiotics, including TC and anhydrotetracycline, have been found to enhance specific binding of low density lipoprotein (LDL) to both LDL receptor-positive and-negative cells at relatively higher concentrations. When incubated at 37°C, the ability of LDL receptor-negative human fibroblasts to bind 125I-LDL was increased from<2 to 45 ng/mg by 170 μM TC. In normal human fibroblasts and Hep G2 cells, 125I-LDL binding was elevated 1.4- to 2-fold by 113 μM TC. The 125I-LDL binding in the presence of TC was diminished by both heparin and EDTA. The enhancement by TC was not observed when 125I-LDL binding was assayed at 4°C. TC enhanced LDL binding to paraformaldehyde-fixed Hep G2 cells, excluding LDL receptor induction in the mechanism. These results demonstrated that TC enhanced cellular LDL binding through a process not involving functional LDL receptors.

Akira Endo

Papers

Organophosphoric acid compound metal salt and remedy for hyperlipemia containing same

Inhibition of Glucan Synthesis by Casein Polymers Crosslinked by Glutaraldehyde.

Metal salt of organic phosphate and an antihyperlipemic agent containing the same

Basic and Developmental Studies of Inhibitors of Cholesterol Biosynthesis

Enhancement of low density lipoprotein binding to both low density lipoprotein receptor-positive and- negative cells by tetracycline antibiotics