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Alexander V. Vlassov
Researcher at Life Technologies
Publications - 52
Citations - 4746
Alexander V. Vlassov is an academic researcher from Life Technologies. The author has contributed to research in topics: RNA & Small interfering RNA. The author has an hindex of 27, co-authored 52 publications receiving 4028 citations. Previous affiliations of Alexander V. Vlassov include Applied Biosystems & University of California.
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Exosomes: Current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials
TL;DR: Strategies for exosome isolation, the understanding to date of exosomes composition, functions, and pathways, and their potential for diagnostic and therapeutic applications are summarized.
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Isolation of Extracellular Vesicles: General Methodologies and Latest Trends.
TL;DR: This review consolidates the data on the classical and state-of-the-art methods for isolation of EVs, including exosomes, highlighting the advantages and disadvantages of each method.
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Lipid-assisted Synthesis of RNA-like Polymers from Mononucleotides
Sudha Rajamani,Alexander V. Vlassov,Seico Benner,Amy Coombs,Felix Olasagasti,David W. Deamer +5 more
TL;DR: This work shows that RNA-like polymers can be synthesized non-enzymatically from mononucleotides in lipid environments, and is the first such application of a nanopore instrument to detect RNA synthesis under simulated prebiotic conditions.
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Distinct microRNA Expression Profiles in Prostate Cancer Stem/Progenitor Cells and Tumor-Suppressive Functions of let-7
TL;DR: In this article, the authors used quantitative real-time-PCR to define miRNA expression patterns in various stem/progenitor cell populations in prostate cancer, including CD44 +, CD133 +, integrin α2β1 +, and side population cells.
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The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo
Jeoffrey Schageman,Emily Zeringer,Mu Li,Tim Barta,Kristi Lea,Jian Gu,Susan Magdaleno,Robert Setterquist,Alexander V. Vlassov +8 more
TL;DR: A complete exosome workflow solution, starting from fast and efficient extraction of exosomes from cell culture media and serum to isolation of RNA followed by characterization ofExosomal RNA content using qRT-PCR and next-generation sequencing techniques is described.