Alison J. North

TL;DR: This protocol allows users to generate high-quality SIM data while accounting and correcting for common artifacts, and allows researchers from students to imaging professionals to create an optimal SIM imaging environment regardless of specimen type or structure of interest.

TL;DR: Some of the most common practical pitfalls faced by researchers during image acquisition are reviewed, and how they can affect the interpretation of the experimental data.

TL;DR: The results support the suggestion that desmosomes have a role in suppression of tumor spreading and provide an experimental demonstration of the functional importance of the cell adhesion recognition sites of desmocollin and desmoglein.

TL;DR: This tutorial and the accompanying poster provide a guide for performing quantitative fluorescence imaging using confocal microscopy, including advice and troubleshooting information from sample preparation and microscope setup to data analysis and statistics.

TL;DR: Investigation of how the virus hijacks cellular proteins to enable the release of virions from an infected cell shows with high temporal and spatial resolution that components of the host endosomal sorting complex required for transport (ESCRT) machinery are recruited to the neck of the assembling virus to facilitate scission of the link between the virus and the cell.