Ana González-Antuña

TL;DR: It is shown that bias remains the major component of the combined uncertainty because of possible problems associated with the implementation of traceability, and some manufacturers have clearly improved their calibration protocols relative to ERM-DA471, but most of them failed to meet the criteria for acceptable CysC measurements.

TL;DR: The main elements and the difficulties of the automated and semi-automated methods for 25-hydroxyvitamin D, from sample preparation to the analytical phase, as well as those related to mass spectrometry are reviewed.

TL;DR: The results demonstrate that the proposed methodology based on the use of (13)C1-labeled standards and IPD is a reliable approach for accurate LC-MS quantitation of small molecules and compatible with full-scan high-resolution mass spectrometry.

TL;DR: A procedure for Stable Isotope Dilution Analysis in molecular Mass Spectrometry which does not require a methodological calibration graph and can be applied in combination with minimal labelling has been evaluated, and a full uncertainty budget determination has been developed taking into account all uncertainty sources.

TL;DR: Cystatin C measurement by isotope dilution mass spectrometry is developed in this study with the aim of making available this tool to support diagnostics of kidney function in the same way.