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Ana Ramos
Researcher at University of Porto
Publications - 45
Citations - 2170
Ana Ramos is an academic researcher from University of Porto. The author has contributed to research in topics: Lactococcus lactis & Lactate dehydrogenase. The author has an hindex of 23, co-authored 39 publications receiving 2040 citations. Previous affiliations of Ana Ramos include Escola Superior Agrária de Coimbra & Universidade Nova de Lisboa.
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Journal ArticleDOI
Comparative study of the thermostabilizing properties of mannosylglycerate and other compatible solutes on model enzymes.
TL;DR: The superior ability of mannosylglycerate to prevent LDH inactivation was accompanied by a higher efficiency in preventing LDH aggregation induced by heat stress, and it is conceivable that it also fulfills a protein thermoprotective function in vivo.
Journal ArticleDOI
Is the glycolytic flux in Lactococcus lactis primarily controlled by the redox charge? Kinetics of NAD(+) and NADH pools determined in vivo by 13C NMR.
Ana Rute Neves,Rita Ventura,Nahla Mansour,Claire Shearman,Michael J. Gasson,Christopher D. Maycock,Ana Ramos,Helena Santos +7 more
TL;DR: The data are interpreted as showing that the glycolytic flux in wild type L. lactis is not primarily controlled at the level of glyceraldehyde-3-phosphate dehydrogenase by NADH, and the ATP/ADP/P(i) content could play an important role.
Journal ArticleDOI
Stabilization of Enzymes against Thermal Stress and Freeze-Drying by Mannosylglycerate.
Ana Ramos,Neil D.H. Raven,Richard J. Sharp,Simonetta Bartolucci,M. Rossi,Raffaele Cannio,J.H.G. Lebbink,J. van der Oost,W.M. de Vos,Helena Santos +9 more
TL;DR: The data show 2-O-(beta)-mannosylglycerate to be a potential enzyme stabilizer in biotechnological applications and suggests a role for this compound as a protein thermostabilizer under physiological conditions.
Journal ArticleDOI
Functional analysis of the Lactococcus lactis galU and galE genes and their impact on sugar nucleotide and exopolysaccharide biosynthesis.
TL;DR: The results indicated that the endogenous GalE activity is not limiting and that the GalU activity level in wild-type cells controls the biosynthesis of intracellular UDP-glucose and UDP-galactose.
Journal ArticleDOI
In vivo nuclear magnetic resonance studies of glycolytic kinetics in Lactococcus lactis.
Ana Rute Neves,Ana Ramos,M C Nunes,Michiel Kleerebezem,J. Hugenholtz,W.M. de Vos,Jonas S. Almeida,Helena Santos +7 more
TL;DR: The model was capable of predicting qualitative shifts in the metabolism of glucose when changing from anaerobic to aerobic conditions and rationalization of the dynamics of glucose metabolism as being driven largely by ATP surplus.