Showing papers by "Anna Rita Migliaccio published in 1988"
TL;DR: The results suggest that the colonies induced by IL-3, GM-CSF, and G- CSF are derived from different progenitors.
106 citations
TL;DR: IL-3 exerts a differentiative and proliferative effect on early stem and progenitor cells, which is concentration dependent, and acts synergistically to induce both proliferation of stem cells and differentiation of erythroid progenitors.
73 citations
TL;DR: Embolic and adult erythroid progenitors differ at least in terms of in vitro sensitivity to GM-CSF and IFN-gamma and suggest that different cellular response to control signals may underlie the differences observed in vivo between embryonic and adult hemopoiesis.
61 citations
TL;DR: It is reported that this ER activity requires interaction with hormone and specific DNA sequence, and this receptor also interacts with calmodulin (CaM), the major intracellular mediator of Ca2+ action in eucaryotic cells.
Abstract: Estradiol receptor (ER) activity requires interaction with hormone and specific DNA sequence. We now report that this receptor also interacts with calmodulin (CaM), the major intracellular mediator of Ca2+ action in eucaryotic cells. This interaction has been observed using both CaM-Sepharose and [125l]CaM. Crude and purified [3H]ER complex show high affinity interaction with CaM-Sepharose [dissociation constant (Kd) 0.12 and 0.16 nm, respectively]. Unoccupied receptor shows a similar high affinity interaction. Tamoxifen-ER complex also binds to CaMSepharose. Several findings show that this CaM-ER interaction is very specific: lack of this interaction has been observed in the presence of trifluoperazine, an inhibitor of protein binding to CaM; the receptor binds neither Sepharose, nor parvalbumin-Sepharose; competition of interaction of [3H]ER complex with CaM-Sepharose is observed by cold ER complex; rat liver glucocorticoid receptor does not bind to CaM-Sepharose. The interaction of purified receptor wi...
57 citations
TL;DR: Investigation of the effects of recombinant interleukin-3, granulocyte-macrophage and granulocytes colony-stimulating factors, and erythropoietin on the number of human hematopoietic progenitors after two to ten days of incubation in liquid cultures deprived of fetal bovine serum indicates that IL-3 alone is capable of increasing thenumber of BFU-E and of maintaining the number in liquid culture.
31 citations
01 Jan 1988
TL;DR: Neither in the case of glucocorticoid receptor nor in the cases of other steroid receptors was it proved that the observed fluctuations of hormone binding were due to phosphorylation-dephosphorylation of either receptors or receptor-related proteins.
Abstract: Our group has been working since 1979 on the process which reversibly regulates hormone binding of the uterus estradiol receptor. The possibility that phosphorylation of proteins is responsible for the hormone binding to steroid receptors was proposed for the first time for the glucocorticoid receptor when it was observed that ATP shortage of thymocytes decreases their ability to bind hormone whereas ATP recovery parallels recovery of this binding (1). Subsequently, it was observed that hormone binding to different steroid receptors is inactivated by exogenous phosphatases and reactivated by processes requiring ATP (2–4). Unfortunately, neither in the case of glucocorticoid receptor nor in the case of other steroid receptors was it proved that the observed fluctuations of hormone binding were due to phosphorylation-dephosphorylation of either receptors or receptor-related proteins.