测定：FVC需要测试者尽力呼出肺内气体，这一过程约需20s，老年人或有严重肺疾病患者的体力常不堪耐受。常规的FVC测定值也会因气道阻塞者或健康高龄人的呼气时间而受影响。作者使用6s内用力呼气量(FEV6.0)代替FVC作为肺功能指标，探讨其对诊断气道阻塞和呼吸受限的优越性。

用FEV6．0代替FVC诊断气道阻塞和肺功能受限[英]／Swanney MP…∥Am J Respir Crit Care Med．

Mutation of the Maturase Lipoprotein Attenuates the Virulence of Streptococcus equi to a Greater Extent than Does Loss of General Lipoprotein Lipidation Andrea Hamilton,†‡ Carl Robinson,† Iain C. Sutcliffe, Josh Slater, Duncan J. Maskell, Nick Davis-Poynter, Ken Smith, Andrew Waller, and Dean J. Harrington* University of Sunderland, SR1 3SD Tyne and Wear, United Kingdom; Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, United Kingdom; Northumbria University, Newcastle upon Tyne NE1 8ST, United Kingdom; Royal Veterinary College, Hertfordshire AL9 7TA, United Kingdom; Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom; and University of Bradford, West Yorkshire BD7 1DP, United Kingdom

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Infection and immunity

Antibiotic resistance in major rivers in the world: A systematic review on occurrence, emergence, and management strategies

Antimicrobial potential of bacteriocins: in therapy, agriculture and food preservation.

Wood Chemistry, Fundamentals and Applications, Second Edition, examines the basic principles of wood chemistry and its potential applications to pulping and papermaking, wood and wood waste utilization, pulping by-products for production of chemicals and energy, and biomass conversion.

Wood chemistry: fundamentals and applications.

Treatment of infectious disease: beyond antibiotics.

Assessment of the metabolic capacity and adaptability of aromatic hydrocarbon degrading strain Pseudomonas putida CSV86 in aerobic chemostat culture.

Cytochrome P450 mono-oxygenases (2UUQ) enzyme from Mycobacterium tuberculosis catalyzes oxidation of organic compounds such as lipids and steroidal hormones therefore remain as potential drug target. Currently available first line anti-tuberculosis drugs have been caused several side effects in the body as well as resistance development by mycobacterium against these drugs, necessitates the considerable need for finding new drugs. Therefore, we propose a structure based computational method to find a new potential inhibitor for cytochrome P450 mono-oxygenases enzyme. Compounds from several ligand databases were docked against the functional sites of 2UUQ (A) through the standard GEMDOCK v2.0 and AUTODOCK4.0 molecular docking tools. Commercially available chemical compound ZINC00004165 (5-[3-(2-nitroimidazol-1-yl) propyl] phenanthridine) has produced top rank with lowest interaction energy of -113.2 (via GEMDOCK) and lowest docking energy of -9.80 kcal/mol (via AUTODOCK) as compared to first line anti TB compounds. Z score and normal distribution analysis verified that the ZINC00004165 compound has more affinity towards 2UUQ in comparison to large number of random population of compounds. ZINC00004165 is also in agreement with the drug likeness properties of Lipinski rule of five without any violation. Therefore, our finding concludes that the commercial compound ZINC00004165 can act as a potential inhibitor against cytochrome P450 mono-oxygenases enzyme of Mycobacterium tuberculosis.

http://www.bioinformation.net/008/97320630008931.pdf

Computational finding of potential inhibitor for Cytochrome P450 Mono-oxygenases Enzyme of Mycobacterium tuberculosis.

The novel 3-methyladenine DNA glycosylase enzyme from oral pathogen Streptococcus sanguinisin involves in DNA repair mechanisms and participates in base excision repair. Its 3D structure is still unknown which may be a potential drug target, therefore here we proposed its putative 3D structure by homology modeling approach. EsyPred3d software produced more precise modeled structure as compare to Swiss model software. The modeled structure was further verified by PROCHECK analysis and subjected to functional site prediction servers for active site residues prediction. The functional site was further validated by molecular docking approach with ligand EDA (3- [2- Deoxyribofuranosyl] - 3H- 1, 3, 4, 5A, 8-Pentaaza- Asindacene-5- monophosphate) from 1F4R. The EDR docked at the cavity of modeled structure of 3-methyladenine DNA glycosylase enzyme with highest Patchdock score of 3966 and lowest Autodock 4 docking energy of -10.30 Kcal/mol. The YA51, LA105, RA107 residues are surrounding the EDA and matching with ligand binding residues predicted by PROFUNC server.

Anshul Nigam

Papers

Treatment of infectious disease: beyond antibiotics.

Assessment of the metabolic capacity and adaptability of aromatic hydrocarbon degrading strain Pseudomonas putida CSV86 in aerobic chemostat culture.

Computational finding of potential inhibitor for Cytochrome P450 Mono-oxygenases Enzyme of Mycobacterium tuberculosis.

Structure modeling of novel DNA glycosylase enzyme from oral pathogen Streptococcus sanguinis.

Dynamics of rate limiting enzymes involved in the sequential substrate uptake by Pseudomonas putida CSV86: Modeling and experimental validation