Showing papers by "Asha J. Dissanayake published in 2021"
TL;DR: Wang et al. as mentioned in this paper used the nuclear ribosomal internal transcribed spacer (ITS), partial sequences of translation elongation factor 1-α (tef1), β-tubulin (tub2), and partial calmodulin (cal) gene regions were sequenced and employed to construct phylogenetic trees.
Abstract: Diaporthe species are associated with Citrus as endophytes, pathogens, and saprobes worldwide. However, little is known about Diaporthe as endophytes in Citrus grandis in China. In this study, 24 endophytic Diaporthe isolates were obtained from cultivated C. grandis cv. "Tomentosa" in Huazhou, Guangdong Province in 2019. The nuclear ribosomal internal transcribed spacer (ITS), partial sequences of translation elongation factor 1-α (tef1), β-tubulin (tub2), and partial calmodulin (cal) gene regions were sequenced and employed to construct phylogenetic trees. Based on morphology and combined multigene phylogeny, eleven Diaporthe species were identified including two new species, Diaporthe endocitricola and D. guangdongensis. These are the first report of D. apiculata, D. aquatica, D. arecae, D. biconispora, D. limonicola, D. masirevicii, D. passifloricola, D. perseae, and D. sennae on C. grandis. This study provides the first intensive study of endophytic Diaporthe species on C. grandis cv. tomentosa in China. These results will improve the current knowledge of Diaporthe species associated with C. grandis. The results obtained in this study will also help to understand the potential pathogens and biocontrol agents and to develop a platform in disease management.
16 citations
TL;DR: In this study, five aquatic hyphomycetous taxa were collected and isolated from submerged wood in Lancang River located in the northwestern Yunnan Province of China and can be recognized as four species which two are introduced as new species (Distoseptispora euseptatensis and D. yunnanensis).
Abstract: In this study, five aquatic hyphomycetous taxa were collected and isolated from submerged wood in Lancang River located in the northwestern Yunnan Province of China. Phylogenetic analyses based on combined LSU, ITS, tef1α and rpb2 sequence matrix indicated that those five taxa belonging to Distoseptispora (Distoseptisporaceae), and they can be recognized as four species which two are introduced as new species (Distoseptispora euseptatensis and D. yunnanensis) and two are identified as D. aquatica and D. tectonae. Two isolates of the new species Distoseptispora euseptatensis formed a distinct clade among Distoseptispora species (basal to D. suoluoensis and D. bambusae) with good support (ML 100%, MP 100%, BI 1.00 PP). Distoseptispora yunnanensis clustered together with D. euseptatensis, but they are difference in the size of conidiophore and conidia. Corresponding illustrations, descriptions and phylogenetic evidences are provided to justify the placement of our newly collected Distoseptispora taxa.
10 citations
01 Jan 2021
8 citations
TL;DR: Morphological characteristics and phylogenetic analyses support the establishment of a novel species, Lasiodiplodia chiangraiensis, isolated from woody hosts, and the accepted genera in Botryosphaeriaceae based on recent studies are given.
Abstract: Lasiodiplodia species are commonly as endophytes, saprobes and pathogens in tropics and subtropics. During an investigation of Botryosphaeriaceae in Thailand, two Lasiodiplodia taxa were isolated. Morphological characteristics and phylogenetic analyses based on combined ITS, tef and tub2 sequence data support the establishment of a novel species, Lasiodiplodia chiangraiensis, isolated from woody hosts. Lasiodiplodia chiangraiensis is phylogenetically close to L. iraniensis and L. thailandica, but represents a distinct lineage. The new species could be distinguished from extant Lasiodiplodia species by its mature conidial dimensions. A detailed description and illustration are provided, as well as an updated phylogenetic tree (ITS, tef and tub2) including all species (with available molecular data) of Lasiodiplodia. In addition, the accepted genera in Botryosphaeriaceae based on recent studies are given.
6 citations
TL;DR: In this paper, 60 saprobic isolates were obtained from decaying woody hosts in southwestern China, and available DNA sequence data was used to infer phylogenetic analyses based on the internal transcribed spacer (ITS), large subunit rRNA gene (LSU), and translation elongation factor 1-α (tef) loci.
Abstract: Botryosphaeriales is an important order of diverse fungal pathogens, saprobes, and endophytes distributed worldwide. Recent studies of Botryosphaeriales in China have discovered a broad range of species, some of which have not been formerly described. In this study, 60 saprobic isolates were obtained from decaying woody hosts in southwestern China. The isolates were compared with other species using morphological characteristics, and available DNA sequence data was used to infer phylogenetic analyses based on the internal transcribed spacer (ITS), large subunit rRNA gene (LSU), and translation elongation factor 1-α (tef) loci. Three novel species were illustrated and described as Botryobambusa guizhouensis, Sardiniella elliptica, and Sphaeropsis guizhouensis, which belong to rarely identified genera within Botryosphaeriaceae. Botryobambusa guizhouensis is the second species identified from the respective monotypic genus. The previously known species were identified as Aplosporella hesperidica, Barriopsis tectonae, Botryosphaeria dothidea, Diplodia mutila, Di. neojuniperi, Di. pseudoseriata, Di. sapinea, Di. seriata, Dothiorella sarmentorum, Do. yunnana, Lasiodiplodia pseudotheobromae, Neofusicoccum parvum, Sardiniella celtidis, Sa. guizhouensis, and Sphaeropsis citrigena. The results of this study indicate that numerous species of Botryosphaeriales are yet to be revealed in southwestern China.
4 citations
TL;DR: This study reveals the first sexual morph of Sardiniella, which it is characterized by 2–4(–6)-spored asci with hyaline to brown, aseptate to 1- septate, ovate to subclavate ascospores.
Abstract: During an investigation of ascomycetous fungi in Karst formations of the Asian region, three interesting taxa were found on dead aerial stems of woody hosts in Guizhou province, China. Their morphology assigned them as typical botryosphaeriaceous species. Phylogenetic analyses based on a combined DNA dataset of large subunit (LSU), internal transcribed spacer (ITS) and part of the translation elongation factor 1-α (tef1) gene confirmed their placement within Botryosphaeriaceae. In the phylogenetic tree, the three isolates formed a well-supported monotypic clade as a distinct lineage within the genus Sardiniella. Therefore, a new species Sardiniella guizhouensis sp. nov. is introduced to accommodate these taxa and detailed, illustrated descriptions of the asexual and sexual morphs are provided. This study reveals the first sexual morph of Sardiniella, which it is characterized by 2–4(–6)-spored asci with hyaline to brown, aseptate to 1-septate, ovate to subclavate ascospores.
3 citations