Showing papers by "Aydin Tozeren published in 1996"
TL;DR: Investigating trauma-induced leukocyte rolling in venules of the exteriorized rat mesentery concludes that selectin-mediated adhesion during rolling produces adhesion energy densities comparable to those observed for integrin- mediated adhesion events in other experimental systems.
Abstract: Leukocyte rolling along the endothelium in inflammation is caused by continuous formation and breakage of bonds between selectin adhesion molecules and their ligands We investigated trauma-induced leukocyte rolling in venules (diameter, 23 to 58 μm; wall shear stress, 12 to 35 dyne/cm2) of the exteriorized rat mesentery using high-resolution intravital microscopy While rolling, the leukocytes deformed into a teardroplike shape Deformation continued to increase with shear stress up to the highest values observed (35 dyne/cm2) Successive leukocytes had similar rolling velocities at the same axial positions along each vessel, suggesting that heterogeneity of endothelial adhesiveness is responsible for velocity variation Adhesion energy density varied inversely with instantaneous rolling velocity and directly with instantaneous deformation Adhesion energy density reached a maximum of 036 dyne/cm, similar to values found for lymphocyte function–associated antigen-1–dependent adhesion of stimulated T ce
115 citations
TL;DR: A finite-difference scheme was used to evaluate the three-dimensional laminar flow past an array of uniformly distributed cells that are adherent to the bottom plate of a parallel-plate flow channel and indicated that fluid force on a spherical cell can be computed within 10% accuracy by using the solution given by Goldman et al.
66 citations
TL;DR: A role for beta-catenin and serine kinase activity in mediating the action of 9-cis-RA in epithelial differentiation is pointed to.
Abstract: In this study we show that a breast cancer cell line (SKBR3) that expresses no E-cadherin and very low levels of beta-catenin protein and exhibits a poorly adhesive phenotype in Matrigel responds to retinoic acid (RA) by a marked increase in epithelial differentiation. Specifically, treatment of cells with all-trans-RA, 9-cis-RA, or a RA receptor alpha-specific ligand resulted in a large increase in cell-cell adhesive strength and stimulated the formation of fused cell aggregates in Matrigel. A retinoid X receptor-specific ligand was ineffective. Exposure of cells to 9-cis-RA for as little as 4 h was sufficient to maintain the adhesive phenotype for at least 4 days. The effects of 9-cis-RA required protein and RNA synthesis, but were not mediated by factors secreted by stimulated cells or by direct cell contact and did not require serum. These 9-cis-RA-induced morphological effects were completely reversed by growing cells in 50 microM Ca2+, suggesting a mechanism involving a 9-cis-RA-induced increase in ...
48 citations