A successful mating involves the coordination of many physiological events including insemination, gamete transport and ovulation. As more basic information about these processes becomes available, it is apparent that there are opportunities for manipulating these physiological events and enhancing the reproductive success of natural and artificial matings. The primary intent of this review is to examine the effectiveness of several of these strategies, as determined by their influence on farrowing rate and litter size. It is evident that the timing, duration and pattern of ovulation in pigs are extremely variable. Thus, increasing the frequency and changing the timing of matings during oestrus has received renewed interest. In general, if a female exhibits oestrus for more than 1 day, then increasing the frequency of matings has a greater influence on reproductive performance than does altering the timing of matings. In addition, litter size is more responsive to increased mating frequencies than is farrowing rate. Increasing the number of matings per oestrus via the use of combinations of natural service and artificial insemination, compared with the use of either alone, appears to enhance reproductive performance. It has been well documented that several aspects of the mating process itself can advance the onset of ovulation and enhance sperm transport and storage in the female reproductive tract. As a result, the use of these stimuli in conjunction with natural and artificial matings provide opportunities for enhancing fecundity. Pre- and post-breeding treatments with vasectomized boars, nonviable semen, oestrogens and seminal plasma have all been reported to increase farrowing rate and litter size. With artificial insemination, additions of prostaglandins, oxytocin, oestrogens and leucocytes to semen have been reported to enhance reproductive performance, albeit with varying degrees of success. Personnel and boar needs are important components in optimizing breeding management. Estimates of these needs for artificial and natural matings are discussed.

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Optimizing management of natural and artificial matings in swine

Bovine tuberculosis (TB) continues to be an intractable problem in many countries, particularly where “test and slaughter” policies cannot be implemented or where wildlife reservoirs of Mycobacterium bovis infection serve as a recurrent source of infection for domestic livestock. Alternative control measures are urgently required and vaccination is a promising option. Although the M. bovis bacille Calmette-Guerin (BCG) vaccine has been used in humans for nearly a century, its use in animals has been limited, principally as protection against TB has been incomplete and vaccination may result in animals reacting in the tuberculin skin test. Valuable insights have been gained over the past 25
years to optimise protection induced by BCG vaccine in animals and in the development of tests to differentiate infected from vaccinated animals (DIVA). This review examines factors affecting the efficacy of BCG vaccine in cattle, recent field trials, use of DIVA tests and the effectiveness of BCG vaccine in other domestic livestock as well as in wildlife. Oral delivery of BCG vaccine to wildlife reservoirs of infection such as European badgers, brushtail possums, wild boar, and deer has been shown to induce protection against TB and could prove to be a practical means to vaccinate these species at scale. Testing of BCG vaccine in a wide range of animal species has indicated that it is safe and vaccination has the potential to be a valuable tool to assist in the control of TB in both domestic livestock and wildlife.

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Efficacy and Safety of BCG Vaccine for Control of Tuberculosis in Domestic Livestock and Wildlife.

Bioinspired intelligent algorithm (BIA) is a kind of intelligent computing method, which is with a more lifelike biological working mechanism than other types. BIAs have made significant progress in both understanding of the neuroscience and biological systems and applying to various fields.Mobile robot control is one of the main application fields of BIAs which has attractedmore and more attention, because mobile robots can be used widely and general artificial intelligent algorithms meet a development bottleneck in this field, such as complex computing and the dependence on high-precision sensors. This paper presents a survey of recent research in BIAs, which focuses on the research in the realization of various BIAs based on different working mechanisms and the applications for mobile robot control, to help in understanding BIAs comprehensively and clearly. The survey has four primary parts: a classification of BIAs fromthe biomimetic mechanism, a summary of several typical BIAs from different levels, an overview of current applications of BIAs in mobile robot control, and a description of some possible future directions for research.

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Bioinspired intelligent algorithm and its applications for mobile robot control: a survey

Bio-load and bio-profile of Mycobacterium avium subspecies paratuberculosis was studied in the domestic livestock population of the country. Of the 23,429 farm and farmer's animals screened, average bio-load was 23.3% (Period of study; 28 years for goats; 13 years for sheep, cattle and buffaloes). Species-wise, bio-load was 20.1, 32.7, 39.3 and 28.3% in goats, sheep, cattle and buffaloes, respectively. Bio-load was significantly lower in time period A (P < 0.001) and B (P < 0.03), compared with period C. Geographical zone-wise, bio-load of MAP was significantly higher (P < 0.05) in Central zone compared with South, West, East and North zones. Bio-load in 11 states ranged from 16.2 to 87.8%. Of 8450, 5643, 8185 and 1151 samples screened by microscopy, culture, indigenous ELISA and IS900 blood PCR, 20.0, 10.6, 35.1 and 26.6% samples were positive, respectively. Bio-load was 32.8 and 31.6% in farm and farmer's goats and sheep, respectively, and 62.1% in farmer's cattle. MAP bio-load was also monitored in four farm units (three goats and one sheep) for breed improvement and three farm goats units for experimental purposes at Central Institute for Research on Goats in Mathura district. Of the 8025 goats and 1525 sheep that died from 1988 to 2013, 10.9 and 3.0% deaths were due to JD, respectively. On the basis of JD and suspected JD, 10.0 and 28.4% goats and 2.2 and 40.9% sheep, respectively were culled from the farm units in 25 years. Microscopic examination of 214 tissues (mesenteric lymph nodes and intestines) of 107 animals, it was observed that bio-load of MAP was high (25.0-60.0%) in farm animals. 'Indian Bison Type' was the dominant biotype, irrespective of domestic livestock species and the geographical zone.

'Bio-Load' and Bio-Type Profiles of Mycobacterium avium subspecies paratuberculosis Infection in the Domestic Livestock Population Endemic for Johne's Disease: A Survey of 28 years (1985-2013) in India

Bovine tuberculosis (bTB) is a chronic disease of cattle that impacts productivity and represents a major public health threat. Despite the considerable economic costs and zoonotic risk consequences associated with the disease, accurate estimates of bTB prevalence are lacking in many countries, including India, where national control programmes are not yet implemented and the disease is considered endemic. To address this critical knowledge gap, we performed a systematic review of the literature and a meta-analysis to estimate bTB prevalence in cattle in India and provide a foundation for the future formulation of rational disease control strategies and the accurate assessment of economic and health impact risks. The literature search was performed in accordance with PRISMA guidelines and identified 285 cross-sectional studies on bTB in cattle in India across four electronic databases and handpicked publications. Of these, 44 articles were included, contributing a total of 82,419 cows and buffaloes across 18 states and one union territory in India. Based on a random-effects (RE) meta-regression model, the analysis revealed a pooled prevalence estimate of 7.3% (95% CI: 5.6, 9.5), indicating that there may be an estimated 21.8 million (95% CI: 16.6, 28.4) infected cattle in India-a population greater than the total number of dairy cows in the United States. The analyses further suggest that production system, species, breed, study location, diagnostic technique, sample size and study period are likely moderators of bTB prevalence in India and need to be considered when developing future disease surveillance and control programmes. Taken together with the projected increase in intensification of dairy production and the subsequent increase in the likelihood of zoonotic transmission, the results of our study suggest that attempts to eliminate tuberculosis from humans will require simultaneous consideration of bTB control in cattle population in countries such as India.

https://onlinelibrary.wiley.com/doi/pdf/10.1111/tbed.12915

Prevalence of Bovine Tuberculosis in India: A systematic review and meta-analysis

Molecular identification of Mycobacterium tuberculosis in cattle.

Ovine paratuberculosis is a threat to small animal holders in terms of economic loss such as reduced growth performance and early culling. In order to study the slaughterhouse prevalence of ovine paratuberculosis, the slaughterhouse sheep samples (which are poor in body condition) collected over a period of two and half years from 1,034 suspected male sheep (poor in body condition) and 40 normal sheep (good body condition and subsequently negative by all the diagnostic tests employed) aged between 16 and 18 months were slaughtered at various abattoirs of Tamil Nadu. All the sheep taken in this study were maintained in almost same management conditions. DNA was extracted from 1,034 intestinal tissue and mesenteric lymph node and 121 were positive by IS 900 PCR. One hundred ten and 56 were positive by absorbed ELISA and Ziehl–Neelsen staining, respectively. In histopathology, 28 animals showed gross lesions of paratuberculosis infection (20—multibacillary and 8—paucibacillary forms). Out of 1,034 sheep tissues cultured, 32 showed cultural growth in Middlebrook 7H9 and 26 in Herrold's egg yolk medium. None of the 40 normal sheep were positive by any of the tests employed. In general, the mean body weight of paratuberculosis-affected animal either by any one of the tests employed was less than the non-affected sheep. The approximate economic loss per sheep/farmer/year is around Rs 1,840 (US$ 38.33) in paratuberculosis-affected sheep.

Slaughterhouse prevalence of ovine paratuberculosis in Southern India.

The biological deoxyribonucleic acid (DNA) strand has been increasingly seen as a promising computing unit. A new algorithm is formulated in this paper to design any DNA Boolean operator with molecular beacons (MBs) as its input. Boolean operators realized using the proposed design methodology is presented. The developed operators adopt a uniform representation for logical 0 and 1 for any Boolean operator. The Boolean operators designed in this work employ only a hybridization operation at each stage. Further, this paper for the first time brings out the realization of a binary adder and subtractor using molecular beacons. Simulation results of the DNA-based binary adder and subtractor are given to validate the design.

A novel generalized design methodology and realization of Boolean operations using DNA

Conventional and molecular determination of drug resistance in Mycobacterium tuberculosis and Mycobacterium bovis isolates in cattle.

Aim: To assess the reproductive performance and managemental advantages of Artificial Insemination of swine. Materials and Methods: Large White Yorkshire sows were grouped into two batches (10 each) for AI and natural mating. AI 9 group was given two inseminations with semen extended with Beltsville Thawing Solution (BTS,3 x 10 spermatozoa per dose), at standing heat. Similarly, for natural mating group, triple mating was followed. The reproductive performance was studied after maintaining the sows under optimal managemental conditions. Results: The results indicated that AI and natural mating practices showed 100% conception rate. The litter size of AI group was 8.36±0.28 and that of the natural mating group was 10.6±0.64, that varied with high significance. The still birth rate was 0.7±0.26, which was significantly higher in natural mating group. The pre-weaning mortality was 4.34% and 7.5% for AI group and natural mating group respectively. The time consumed during mating per sow also varied highly significantly which was 11.46±0.47 minutes and 51.2±2.08 minutes for the AI group and natural mating group respectively. Conclusion: It was found that AI was found to be time and labor saving. The reduced litter size in AI group could be improved by ensuring that inseminations occur at an optimum time resulting in a high farrowing rate and litter size. AI can be successfully introduced in field conditions with some fine tuning.

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B.S.M. Ronald

Papers

Molecular identification of Mycobacterium tuberculosis in cattle.

Slaughterhouse prevalence of ovine paratuberculosis in Southern India.

A novel generalized design methodology and realization of Boolean operations using DNA

Conventional and molecular determination of drug resistance in Mycobacterium tuberculosis and Mycobacterium bovis isolates in cattle.

Artificial Insemination in Swine in an Organized Farm – A Pilot Study