Showing papers by "Barbara C. Furie published in 1985"

Immunoassay means and methods useful in human native prothrombin and human abnormal prothrombin determinations

Abstract: Antibodies which form immune complexes with human native prothrombin only, in the presence of mixtures of human native prothrombin and human abnormal prothrombin as well as antibodies which form antibody antigen complexes with human abnormal prothrombin in the presence of such mixtures have been obtained Immunoassay techniques are used for qualitative and quantitative determinations of these antigens in human plasma or serum Unique methods of obtaining the antibodies are described including obtaining antibodies to native prothrombin by dissociation of antigen antibody complexes formed in the presence of calcium ions with a material having a greater affinity constant for binding with calcium ions than does prothrombin Dissociation of the complex in this manner yields human native prothrombin antibodies which are specific and non-reactive with human abnormal prothrombin A process is described in which assays are applied to the sensitive detection of vitamin K deficiency and various forms of liver disease including hepatocellular carcinoma, and to monitoring of anticoagulant therapy with sodium warfarin The invention described herein was made in the course of working under a grant from the Department of Health and Human Services

PART II. COMPUTER‐ASSISTED MACROMOLECULAR STRUCTURE GENERATION: EXTENSION OF EXISTING INFORMATION: On the Construction of Computer Models of Proteins by the Extension of Crystallographic Structures

Abstract: One of the central beliefs of molecular biology is that the sequence of amino acids determines the structure of a p r ~ t e i n . ~ ~ Yet our scientific culture has still to discover many of the rules that specify and govern this relationship. The results of X-ray crystallographic determination of specific proteins have led to an understanding of the taxonomy of protein architecture^.^ The protein sequence fileS which had been growing rather slowly because each amino acid sequence had to be determined by chemical techniques is now growing exponentially because amino acid sequences can be derived from nucleic acid sequencese6 Protein extension attempts to shorten the delay be-