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Benjamin Flusberg
Researcher at Pacific Biosciences
Publications - 26
Citations - 3436
Benjamin Flusberg is an academic researcher from Pacific Biosciences. The author has contributed to research in topics: Nucleic acid & Nanopore sequencing. The author has an hindex of 12, co-authored 26 publications receiving 3167 citations.
Papers
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Journal ArticleDOI
Direct detection of DNA methylation during single-molecule, real-time sequencing
Benjamin Flusberg,Dale R. Webster,Jessica Lee,Kevin Travers,Eric Olivares,Tyson A. Clark,Jonas Korlach,Stephen Turner +7 more
TL;DR: The direct detection of DNA methylation, without bisulfite conversion, through single-molecule, real-time (SMRT) sequencing is described and is amenable to long read lengths and will likely enable mapping of methylation patterns in even highly repetitive genomic regions.
Book ChapterDOI
Real-time DNA sequencing from single polymerase molecules.
Jonas Korlach,Keith Bjornson,Bidhan Chaudhuri,Ronald L. Cicero,Benjamin Flusberg,Jeremy Gray,David P. Holden,Ravi Saxena,Jeffrey Wegener,Stephen Turner +9 more
TL;DR: This chapter will first outline the principle of this single-molecule, real-time (SMRT) DNA sequencing method, followed by descriptions of its underlying components and typical sequencing run conditions.
Journal ArticleDOI
Real-time tRNA transit on single translating ribosomes at codon resolution
Sotaro Uemura,Colin Echeverría Aitken,Colin Echeverría Aitken,Jonas Korlach,Benjamin Flusberg,Stephen Turner,Joseph D. Puglisi +6 more
TL;DR: The results show that ribosomes are only briefly occupied by two tRNA molecules and that release of deacylated tRNA from the exit (E) site is uncoupled from binding of aminoacyl-tRNA site (A-site) tRNA and occurs rapidly after translocation.
Patent
Nanopore sequencing devices and methods
Stephen Turner,Benjamin Flusberg,Mathieu Foquet,Hans Callebaut,Robert Sebra,Bidhan Chaudhuri,Jon M. Sorenson,Keith Bjornson,Adrian Fehr,Jonas Korlach,Robin Emig +10 more
TL;DR: In this paper, the authors described a method for controlling translocation of through the nanopore, which includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS.
Patent
Classification of nucleic acid templates
Benjamin Flusberg,Jonas Korlach,Jeffrey Wegener,Tyson A. Clark,Igor D. Vilfan,Andrey Kislyuk,Stephen Turner,Jon M. Sorenson,Kevin Travers,Cheryl Heiner,Austin B. Tomaney,Patrick Marks,Jessica Lee,Lei Jia,Dale R. Webster,John Lyle,Jeremiah Hanes,Joseph D. Puglisi +17 more
TL;DR: In this article, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. But these methods are not suitable for the characterization of nucleic acids.