R
Ronald L. Cicero
Researcher at Pacific Biosciences
Publications - 11
Citations - 5070
Ronald L. Cicero is an academic researcher from Pacific Biosciences. The author has contributed to research in topics: Single molecule real time sequencing & Multiple displacement amplification. The author has an hindex of 7, co-authored 11 publications receiving 4794 citations. Previous affiliations of Ronald L. Cicero include Life Technologies.
Papers
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Journal ArticleDOI
Real-Time DNA Sequencing from Single Polymerase Molecules
John Eid,Adrian Fehr,Jeremy Gray,Khai Luong,John Lyle,Geoff Otto,Paul Peluso,David R. Rank,Primo Baybayan,Brad Bettman,Arkadiusz Bibillo,Keith Bjornson,Bidhan Chaudhuri,Fred Christians,Ronald L. Cicero,Sonya Clark,Ravindra V. Dalal,Alex DeWinter,John Dixon,Mathieu Foquet,Alfred Gaertner,Paul Hardenbol,Cheryl Heiner,Kevin Hester,David P. Holden,Gregory J. Kearns,Xiangxu Kong,Ronald Kuse,Yves Lacroix,Steven Lin,Paul Lundquist,Congcong Ma,Patrick Marks,Mark Maxham,Devon Murphy,Insil Park,Thang Pham,Michael Phillips,Joy Roy,Robert Sebra,Gene Shen,Jon M. Sorenson,Austin B. Tomaney,Kevin Travers,Mark Trulson,John Vieceli,Jeffrey Wegener,Dawn Wu,Alicia Yang,Denis Zaccarin,Peter Zhao,Frank Zhong,Jonas Korlach,Stephen Turner +53 more
TL;DR: Single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs) are presented.
Book ChapterDOI
Real-time DNA sequencing from single polymerase molecules.
Jonas Korlach,Keith Bjornson,Bidhan Chaudhuri,Ronald L. Cicero,Benjamin Flusberg,Jeremy Gray,David P. Holden,Ravi Saxena,Jeffrey Wegener,Stephen Turner +9 more
TL;DR: This chapter will first outline the principle of this single-molecule, real-time (SMRT) DNA sequencing method, followed by descriptions of its underlying components and typical sequencing run conditions.
Journal ArticleDOI
Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures
Jonas Korlach,Patrick Marks,Ronald L. Cicero,Jeremy Gray,Devon Murphy,Daniel B. Roitman,Thang Pham,Geoff Otto,Mathieu Foquet,Stephen Turner +9 more
TL;DR: Results presented here support the application of immobilized single DNA polymerases in ZMW arrays for long-read-length DNA sequencing.
Patent
Zero-mode waveguides with non-reflecting walls
TL;DR: In this article, the optical containment structures generally comprise zero-mode waveguide structures having non-reflective walls, which allow the preparation of optical containment regions in which the containment dimensions can be decoupled from the solution containment dimensions.
Patent
Modified surfaces for immobilization of active molecules
TL;DR: In this paper, modified surfaces, substrates, and methods of producing and using such substrates and surfaces are provided, which provide either nonreactive surfaces or low density reactive groups, preferably on an otherwise non-reactive surface, for use in different applications including single molecule analyses.