Benoit Devogelaere

TL;DR: Using structure-based design, a 1,5-benzodiazepine NS5B polymerase inhibitor chemotype is optimized into a new sulfone-containing scaffold that yielded potent inhibitor (S)-4c (K(D) = 0.79 nM), which has approximately 20-fold greater affinity for NS 5B than its carbonyl analogue (R)-2c.

TL;DR: 1a/1b subtype profiling of polymerase inhibitors that bind at each of the four known nonnucleoside binding sites is reported, showing that inhibition of all of the clinical isolates tested is maintained, except for inhibitors that binds at the palm-1 binding site.

TL;DR: The methods to express and purify the dengue NS5 polymerase from E. coli are described and a validated high-throughput enzymatic assay for screening inhibitors of NS 5 polymerase is developed for screening compounds for identifying polymerase inhibitors against DENV.

TL;DR: Improved compositions for and methods of processing and analyzing samples are described in this paper, in particular the compositions and methods liberate nucleic acids from a biological sample allowing direct downstream processing of the nucleic acid in microfluidic systems.

TL;DR: The new MDx platform is a fast and reliable method for BRAF V600 mutation testing directly on FFPE tumor shavings with superior analytical sensitivity, ease of use, and turnaround time compared to existing diagnostic tests.