Showing papers by "Bernard M. Babior published in 2006"
TL;DR: Oxygen free radical production in hypertension may be associated with elevated arteriolar tone and organ injury and more detailed understanding of where oxidative enzymes are located in the microcirculation is gained.
Abstract: Objective: Oxygen free radical production in hypertension may be associated with elevated arteriolar tone and organ injury. Previous results suggest an enhanced level of oxygen free radical formation in microvascular endothelium and in circulating neutrophils associated with xanthine oxidase activity in the spontaneously hypertensive rats (SHR) compared with their normotensive controls, the Wistar Kyoto rats (WKY). The aim of this study was to gain more detailed understanding of where oxidative enzymes are located in the microcirculation.Methods: An approach was developed to delineate the cellular distribution of two selected oxidative enzymes, xanthine oxidase and nicotinamide adenine dinucleotide phosphate (NADPH) dependent oxidase (protein 67-kDa fraction). Immunolabeling with peroxidase substrate was utilized, which permits full delineation of the primary antibody in all microvascular structures of the mesentery.Results: Xanthine oxidase is present in the endothelium of all segments of the microcircul...
49 citations