The reduction of HAuCl4 by Na2S has been reported to produce gold nanoparticles with an optical absorption in the near-infrared along with its characteristic absorption in the visible. The optical resonances in the visible are due to the gold surface plasma, which are a function of the geometry of the particles. The near-infrared absorption had been attributed to the formation of Au2S/Au core/shell structures. In this report we present new electronic absorption, electron microscopy, and X-ray absorption data in several systems to show that the near-infrared absorption does not involve core/shell structures. We further suggest that the near-infrared adsorption is most likely the result of the formation of aggregates of gold nanoparticles. The identification of the origin of the near-infrared resonance is critical in understanding the optical properties of metal nanoparticle systems.

Near Infrared Optical Absorption of Gold Nanoparticle Aggregates

Structural-functional analyses of textile dye degrading azoreductase, laccase and peroxidase: a comparative in silico study

Keratinases are considerably gaining momentum in green technology because of their endowed robustness and multifaceted application potentials, such as keratinous agro-wastes valorization. Therefore, the production of novel keratinases from relatively nonpathogenic bacteria grown in agro-wastes formulated medium is cost-effective, and also imperative for the sustainability of thriving bioeconomy. In this study, we optimized keratinase production by Bacillus sp. Nnolim-K1 grown in chicken feather formulated medium. The produced keratinase (KerBNK1) was biochemically characterized and also, the keratinase-encoding gene (kerBNK1) was amplified and sequenced. The optimal physicochemical conditions for extracellular keratinase production determined were 0.8% (w/v) xylose, 1.0% (w/v) feather, and 3.0% (v/v) inoculum size, pH 5.0, temperature (25 °C) and agitation speed (150 rpm). The maximum keratinase activity of 1943.43 ± 0.0 U/mL was achieved after 120 h of fermentation. KerBNK1 was optimally active at pH and temperature of 8.0 and 60 °C, respectively; with remarkable pH and thermal stability. KerBNK1 activity was inhibited by ethylenediamine tetra-acetic acid and 1,10-phenanthroline, suggesting a metallo-keratinase. The amplified kerBNK1 showed a band size of 1104 bp and the nucleotide sequence was submitted to the GenBank with accession number MT268133. Bacillus sp. Nnolim-K1 and the keratinase displayed potentials that demand industrial and biotechnological exploitations.

https://www.mdpi.com/2076-2607/8/9/1304/pdf

Biochemical and Molecular Characterization of a Thermostable Alkaline Metallo-Keratinase from Bacillus sp. Nnolim-K1.

Xylan from bambara and cowpea biomass and their structural elucidation

The high dependency and surplus use of agrochemical products have liberated enormous quantities of toxic halogenated pollutants into the environment and threaten the well-being of humankind. Herein, this study performed molecular docking, molecular dynamic (MD) simulations, molecular mechanics-Poisson Boltzmann Surface Area (MM-PBSA) calculations on the DehH2 from Bacillus thuringiensis, to identify the order of which the enzyme degrades different substrates, haloacids, haloacetate and chlorpyrifos. The study discovered that the DehH2 favored the degradation of haloacids and haloacetates (-3.3 - 4.6 kcal/mol) and formed three hydrogen bonds with Asp125, Arg201 and Lys202. Despite the inconclusive molecular docking result, chlorpyrifos was consistently shown to be the least favored substrate of the DehH2 in MD simulations and MM-PBSA calculations. Results of MD simulations revealed the DehH2-haloacid- (RMSD 0.15 - 0.25 nm) and DehH2-haloacetates (RMSF 0.05 - 0.25 nm) were more stable, with the DehH2-L-2CP complex being the most stable while the least was the DehH2-chlorpyrifos (RMSD 0.295 nm; RMSF 0.05 - 0.59 nm). The Molecular Mechanics Poisson-Boltzmann Surface Area calculations showed the DehH2-L-2CP complex (-24.27 kcal/mol) having the lowest binding energy followed by DehH2-MCA (-22.78 kcal/mol), DehH2-D-2CP (-21.82 kcal/mol), DehH2-3CP (-21.11 kcal/mol), DehH2-2,2-DCP (-18.34 kcal/mol), DehH2-2,3-DCP (-8.34 kcal/mol), DehH2-TCA (-7.62 kcal/mol), while chlorpyrifos was unable to spontaneously bind to DehH2 (+127.16 kcal/mol). In a nutshell, the findings of this study offer valuable insights into the rational tailoring of the DehH2 for expanding its substrate specificity and catalytic activity in the near future.Communicated by Ramaswamy H. Sarma.

Molecular docking and molecular dynamics simulation of Bacillus thuringiensis dehalogenase against haloacids, haloacetates and chlorpyrifos.

In silico studies on bacterial xylanase enzyme: Structural and functional insight

/pdf/biotechnological-potentials-of-halophilic-microorganisms-and-3qw6fxu1.pdf

Biotechnological potentials of halophilic microorganisms and their impact on mankind

Castor being a perennial, cross-pollinated, sexually polymorphic crop with high environmental sensitivity, initial plant breeding efforts were restricted to plant height and duration. Domestication of a wild, perennial crop to an annual crop of medium plant height and duration is the first success. Further, development of a two-line breeding system and standard seed production technology led to successful commercial exploitation of heterosis. Being a monotypic genus, diversification of parental base is restricted to intra-generic, intraspecific, or inter-varietal hybridization. Phenotypic expression is highly plastic and varies with locations and seasons. Majority of the morphological characters are monogenic, independently assorted with very limited linkages among the traits. However, information on genetics of major morphological characters is scattered in several old publications. Conventional breeding methods were successful in developing about 40 high-yielding hybrids and varieties with inbuilt resistance to major pests and diseases. An effort is made in the present chapter to consolidate the information on genetics and breeding methods followed in India and elsewhere.

Classical Genetics, Cytogenetics, and Traditional Breeding in Castor Bean

https://link.springer.com/content/pdf/10.1186/s43141-021-00121-6.pdf

In silico characterization of bacterial chitinase: illuminating its relationship with archaeal and eukaryotic cousins

Contamination of soil by antibiotics and heavy metals originating from hospital facilities has emerged as a major cause for the development of resistant microbes. We collected soil samples surrounding a hospital effluent and measured the resistance of bacterial isolates against multiple antibiotics and heavy metals. One strain BMCSI 3 was found to be sensitive to all tested antibiotics. However, it was resistant to many heavy metals and metalloids like cadmium, chromium, copper, mercury, arsenic, and others. This strain was motile and potentially spore-forming. Whole-genome shotgun assembly of BMCSI 3 produced 4.95 Mb genome with 4,638 protein-coding genes. The taxonomic and phylogenetic analysis revealed it, to be a Bordetella petrii strain. Multiple genomic islands carrying mobile genetic elements; coding for heavy metal resistant genes, response regulators or transcription factors, transporters, and multi-drug efflux pumps were identified from the genome. A comparative genomic analysis of BMCSI 3 with annotated genomes of other free-living B. petrii revealed the presence of multiple transposable elements and several genes involved in stress response and metabolism. This study provides insights into how genomic reorganization and plasticity results in evolution of heavy metals resistance by acquiring genes from its natural environment. 

/pdf/genomic-morphological-and-biochemical-analyses-of-a-multi-3c4nzbrm.pdf

Bhramar Dutta

Papers

In silico studies on bacterial xylanase enzyme: Structural and functional insight

Biotechnological potentials of halophilic microorganisms and their impact on mankind

Classical Genetics, Cytogenetics, and Traditional Breeding in Castor Bean

In silico characterization of bacterial chitinase: illuminating its relationship with archaeal and eukaryotic cousins

Genomic, morphological, and biochemical analyses of a multi-metal resistant but multi-drug susceptible strain of Bordetella petrii from hospital soil