Showing papers in "Journal of Genetic Engineering and Biotechnology in 2021"

TL;DR: A comprehensive review of the structural, physiological, and pharmacological properties of GABAA receptors can be found in this paper, where the authors summarize the current understanding of the structure and physiological properties as well as shedding light on the most common associated disorders.

TL;DR: A review of recent developments of DNA markers and their application in plant breeding programs and devoted to early breeders with little or no knowledge about the DNA markers is presented in this article.

TL;DR: In this paper, a review article explains the different factors that should be considered for the effective synthesis of AgNPs using algae, which can help in modulating the stability and size of the nanoparticles, thereby improving its cost effectiveness and environment-friendly production.

TL;DR: In this paper, a cost-effective method for synthesizing magnesium oxide nanoparticles from Pterocarpus marsupium Roxb heartwood extract with biomedical applications is presented.

TL;DR: Actinomycetes represent a source of biologically active secondary metabolites like antibiotics, biopesticide agents, plant growth hormones, antitumor compounds, antiviral agents, pharmacological compounds, pigments, enzymes, enzyme inhibitors, anti-inflammatory compounds, single-cell protein feed, and biosurfactant as mentioned in this paper.

TL;DR: In this paper, a review summarizes data supporting the importance of exopolysaccharides during biofilm formation and its role in antibiotic resistance and strategies to overcome or attack biofilms are provided.

TL;DR: In this article, an extracellular lipase was partially purified from the culture broth of Rhizopus oryzae R1 isolate to apparent homogeneity using ammonium sulfate precipitation followed by desalting via dialysis.

TL;DR: In this paper, the authors investigated the effect of Pseudomonas sp. on hexavalent chromium (Cr)-resistant rhizobacteria (PGPR) by using classical biochemical tests (phosphate solubilization, nitrogen fixation, indole acetic acid, exopolysaccharides, hydrogen cyanide, siderophores, ammonia, cellulase, pectinase, and chitinase production).

TL;DR: In this paper, the authors determined the relationship among 48 Aegilops triuncialis accessions using three DNA marker systems, including start codon targeted (SCoT), CAAT box-derived polymorphism (CBDP), and inter-simple sequence repeat (ISSR) markers.

TL;DR: A review of the advantages and challenges associated with monoclonal antibody production can be found in this article, which also enlightens the advancement, clinical significance, and future aspects of this technique.

TL;DR: In this article, the structural and functional role of designer nucleases with emphasis on TALENs and CRISPR/Cas9 genome editing system is discussed and a mini review suggests that, TALENS provides novel opportunities in the field of therapeutics being highly specific and sensitive toward DNA modifications.

TL;DR: In this article, the authors demonstrate the modification of the FAD2 gene in rice using a multiplex sgRNA-CRISPR/Cas9 genome editing system, which is a promising tool for plant gene functional studies and crop improvement.

TL;DR: Induced polyploidy serves as an efficient approach in extricating genetic potential of cells and brings about a lot of beneficial changes in plants, coupled with very few detrimental effects as mentioned in this paper.

TL;DR: In this article, 16 derivatives of gallic acid were docked against five selected nonstructural proteins of SARS-COV-2 known to be a good target for finding small molecule inhibitors against the virus.

TL;DR: In this paper, the authors reported a Sanger method-based genome sequence of a viral isolate named SARS-CoV-2 NIB-1, circulating in Bangladesh, which has four non-synonymous mutations in V121D, V843F, A889V, and G1691C positions.

TL;DR: In this article, the authors evaluated the antimicrobial potential of both methanolic and ethanolic extracts of three fruit peels (orange, pomegranate, and banana), against 4 Gram-positive (G+), 3 Gram-negative bacteria (G−), and 2 fungal strains.

TL;DR: The potential salt-tolerant bacteria were screened for their symbiotic heterogeneous bacteria that play an important role in the recycling of plant nutrients through phytostimulation and phytoremediation as mentioned in this paper.

TL;DR: In this article, the potential of anti-HIV reverse transcriptase (RT) phytochemicals as inhibitors of SARS-CoV-2 non-structural proteins (nsps) was explored.

TL;DR: A peptide-based vaccine construct against canine circovirus was designed by joining the selected epitopes with adjuvants by suitable linker, and the cloning and expression of the vaccine construct was also performed using in silico methods as mentioned in this paper.

TL;DR: In this paper, the efficacy of biogenic gold nanoparticles synthesized using Argemone mexicana L. aqueous extract (AM-AuNPs) against the human colon cancer cell line, HCT-15, was investigated.

TL;DR: In this article, the structure and properties of M4 metalloprotease proteins were identified and validated using QMEAN4 and SAVES server (Ramachandran plot, verify 3D, and ERRAT) which proved the stability, reliability and consistency of the tertiary structure of the protein.

TL;DR: In this article, a review of potato growth and development is presented, focusing on the relative roles of hormones during various developmental stages with a view to recognize their mechanisms of function in potato tuber development.

TL;DR: In this paper, the authors used CAAT box-derived polymorphism (CBDP) and start codon targeted (SCoT) markers to estimate molecular variability within the set of 91 samples from Triticum aestivum, Aegilops cylindrica, and Aegilopsis crassa species.

TL;DR: In this paper, the authors used genome-wide association studies, mutational breeding, marker-assisted breeding, double haploid production, hyperspectral imaging, and CRISPR/Cas for dissecting the in-depth physiological mechanisms.

TL;DR: In this paper, a green methodology was used to synthesize copper oxide nanoparticles (CuO NPs) utilizing heartwood aqueous extract of Suaeda maritima (L.) Dumort.

TL;DR: In this paper, a study was carried out to compare the identifying efficacy of maturase K (matK) and ribulose 1,5-biphosphate carboxylase (rbcL) loci on jewel orchid population collected throughout Vietnam.

TL;DR: In this article, the optimal conditions of the fermentation culture medium used for the production of extracellular enzymes (amylase, cellulase, lipase, and protease) from previously isolated Aspergillus niger strains in date by-products were studied.

TL;DR: In this article, the internal transcribed spacer region (ITS) of the rRNA encoding gene and their phylogenetic relationships, antimicrobial activities, and biochemical and phenolic compounds were evaluated.

TL;DR: In this paper, a multi-epitope vaccine against Staphylococcus aureus targeting TSST-1 was designed through an immunoinformatics approach, where B cell and T cell epitopes were predicted in silico and mapped with linkers to avoid junctional immunogenicity and to ensure the efficient presentation of exposed epitopes through HLA.

TL;DR: The study indicates that the marine sponge–associated Streptomyces sp.