Showing papers by "Birbal Singh published in 2022"
3 citations
TL;DR: The AA-SRF at 100 μg dose best supported the LPS/Con A primed splenocyte proliferation activity, elevated the serum OVA-specific total IgG antibody, IL-12, CD4 titer and upsurged CD3/CD19 expression in spleen as well as lymph node sections which in turn advocated its adjuvant potential.
Abstract: Abstract The hemolytic activity, in vitro as well as in vivo toxicity, and immunomodulatory potential of saponins-rich fraction of Asparagus adscendens Roxb. fruit (AA-SRF) have been assessed in this study in order to explore AA-SRF as an alternative safer adjuvant to standard Quil-A saponin. The AA-SRF showed lower hemolytic activity (HD50 = 301.01 ± 1.63 µg/ml) than Quil-A (HD50 = 17.15 ± 2.12 µg/ml). The sulforhodamine B assay also revealed that AA-SRF was less toxic to VERO cells (IC50≥200 ± 4.32 µg/ml) than Quil-A (IC50 = 60 ± 2.78 µg/ml). The AA-SRF did not lead to mortality in mice up to 1.6 mg and was much safer than Quil-A for in vivo use. Conversely, mice were subcutaneously immunized with OVA 100 μg alone or along with Alum (200 μg) or Quil-A (10 μg) or AA-SRF (50 μg/100 μg/200 μg) on days 0 and 14. The AA-SRF at 100 μg dose best supported the LPS/Con A primed splenocyte proliferation activity, elevated the serum OVA-specific total IgG antibody, IL-12, CD4 titer and upsurged CD3/CD19 expression in spleen as well as lymph node sections which in turn advocated its adjuvant potential. Thus, AA-SRF can be further studied for use as a safe alternative adjuvant in vaccines.
1 citations
01 Jan 2022
TL;DR: The somatic cell nuclear transfer (SCNT) has pioneered a new era in cellular medicine and biopharming as discussed by the authors , which is used to generate patient-specific stem cells, thus it avoids dependence on embryos to produce embryonic stem cells (ESCs) which has stern ethical concerns in humans.
Abstract: Genome reprogramming of a cell nucleus to an undifferentiated totipotent or pluripotent cell state provides attractive means of obtaining isogenic immunocompatible cells for therapeutic applications and the organoids for diseases modeling and drug testing. As a potent genome reprogramming tool the somatic cell nuclear transfer (SCNT) has pioneered a new era in cellular medicine and biopharming. SCNT is used to generate patient-specific stem cells, thus it avoids dependence on embryos to produce embryonic stem cells (ESCs) which has stern ethical concerns in humans. In addition, SCNT has facilitated the cost effective large scale production of recombinant biomolecules through cloned transgenic or genetically-modified animals.
TL;DR: In this paper , the authors discuss the state-of-the-art of information about Orientia tsutsugamushi-mediated scrub typhus and delineates the role of omics technologies to develop drugs against the pathogen.
TL;DR: This research presents a novel probabilistic procedure that allows for direct measurement of the response of the immune system to earthquake-triggered landsliding.
Abstract: [This corrects the article DOI: 10.1155/2012/902917.].