Brandi N. Snyder-Talkington

TL;DR: The co-culture system identified that alveolar-capillary exposure to MWCNT induced multiple changes to the underlying endothelium, potentially through cell signaling mediators derived from M WCNT-exposed epithelial cells, and appears to be a relevant in vitro method to study the pulmonary toxicity of MWC NT.

TL;DR: This review proposes that advanced coculture models, combined with integrated analysis of genome-wide in vivo and in vitro toxicogenomic data, may lead to development of predictive multigene expression-based models to better determine toxicity profiles of nanomaterials and consequent potential human health risk due to exposure to these compounds.

TL;DR: It is identified that the novel computational model was sufficient to determine biological processes strongly associated with the pathology of lung inflammation and fibrosis and could identify potential toxicity signaling pathways and mechanisms of MWCNT exposure which could be used for future animal studies to support human risk assessment and intervention efforts.

TL;DR: As coculture gene expression better correlated with in vivo gene expression, it is suggested that cellular cocultures may offer enhanced in vitro models for nanoparticle risk assessment and the reduction of in vivo toxicological testing.

TL;DR: Estimating the regulatory networks between mRNAs and miRNAs in different disease states would be beneficial for understanding the complex mechanisms of pathogenesis may be useful for determining biomarkers of MWCNT-induced lung inflammation and fibrosis for early detection of disease.