Brandon Doyle

TL;DR: The crystal structure of human PRMT5 in complex with MEP50, bound to an S-adenosylmethionine analog and a peptide substrate derived from histone H4 is determined and the structure of the surprising hetero-octameric complex reveals the close interaction between the seven-bladed β-propeller MEP50 and the N-terminal domain ofPRMT5, and delineates the structural elements of substrate recognition.

TL;DR: The results indicate that opalescent appearance is not due to self-association but is a simple consequence of Rayleigh scatter, and did not result in physical instability.

TL;DR: In this paper, PEGylated insulin lispro compounds are defined as insulin compounds with high molecular weight poly(ethylene glycol) and characterized by pharmacokinetic, pharmacodynamic, and/or activity peak-trough ratios of less than 2.

TL;DR: The identification and characterization of this protease enabled the development of a robust purification process by implementation of a controlled temperature inactivation unit operation (heat inactivation) that enabled essentially complete inactivation of the protease, resulting in the production of a stable drug product that had not been possible using column chromatography alone.

TL;DR: The utility of several orthogonal HOS methods as investigational tools during purification process development and the importance of HOS characterization as a component of overall biopharmaceutical analytical control strategy are underlined.