John Michael Beals

TL;DR: The crystal structure at 2.4 Å resolution of a human mutant OB protein (leptin-E100) is reported that has comparable biological activity to wild type but which crystallizes more readily and reveals a four-helix bundle similar to that of the long-chain helical cytokine family.

TL;DR: The deamidation half-life of amino acid residue Asn55 in vivo and the ratio of the deamidated derivatives, i.e., isoAsp55 and Asp55 are determined, which is rapid and sensitive with low-nanogram quantities of protein detected in the biological matrix.

TL;DR: It is hypothesized that the diffusion of the phenolic ligands from the site of injection results in the dissociation of hexamers directly to monomers, thereby maintaining the rapid time-action of the monomeric analog in spite of the hexameric conformation in therapeutic formulations.

TL;DR: LY2405319 exhibited all the biopharmaceutical and biological properties required for initiation of a clinical program designed to test the hypothesis that administration of exogenous FGF21 would result in effects on disease-related metabolic parameters in humans.

TL;DR: The effect of electrostatic forces on EPO binding was studied by measuring binding kinetics in varying NaCl concentrations and it was determined that sialic acid decreased the association rate constant (k(on)) about 3-fold, suggesting that long-range electrostatic interactions are primarily important in determining the rate of association between EPO and EPOR.