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Brandon Klein
Researcher at University of Montana
Publications - 9
Citations - 436
Brandon Klein is an academic researcher from University of Montana. The author has contributed to research in topics: Dried blood spot & Emtricitabine. The author has an hindex of 8, co-authored 9 publications receiving 349 citations. Previous affiliations of Brandon Klein include University of Colorado Denver.
Papers
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Journal ArticleDOI
Determination of nucleoside analog mono-, di-, and tri-phosphates in cellular matrix by solid phase extraction and ultra-sensitive LC-MS/MS detection.
Lane R. Bushman,Jennifer J. Kiser,Joseph E. Rower,Brandon Klein,Jia-Hua Zheng,Michelle L. Ray,Peter L. Anderson +6 more
TL;DR: An ultra-sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) assay was developed and validated to facilitate the assessment of clinical pharmacokinetics of nucleotide analogs from lysed intracellular matrix and was successfully applied to clinical research samples generating novel intrACEllular information for TFV, FTC, ZDV, and 3TC nucleotides.
Journal ArticleDOI
Application of an intracellular assay for determination of tenofovir-diphosphate and emtricitabine-triphosphate from erythrocytes using dried blood spots.
Jia-Hua Zheng,Caitlin Rower,Kevin McAllister,Jose R Castillo-Mancilla,Brandon Klein,Amie L. Meditz,L. Anthony Guida,Jennifer J. Kiser,Lane R. Bushman,Peter L. Anderson +9 more
TL;DR: The described method was applied to HIV prophylaxis studies, demonstrating powerful associations with HIV acquisition through its ability to discriminate gradients of adherence.
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Intracellular Tenofovir and Emtricitabine Anabolites in Genital, Rectal, and Blood Compartments from First Dose to Steady State
Sharon M Seifert,Xinhui Chen,Amie L. Meditz,Jose R Castillo-Mancilla,Edward M. Gardner,Julie A. Predhomme,Carolyn Clayton,Gregory L. Austin,Brent E. Palmer,Jia-Hua Zheng,Brandon Klein,Becky Jo Kerr,L. Anthony Guida,Caitlin Rower,Joseph E. Rower,Jennifer J. Kiser,Lane R. Bushman,Samantha MaWhinney,Peter L. Anderson +18 more
TL;DR: Css values were similar between HIV-negative and HIV-positive individuals, and TFV-DP in rectal mononuclear cells was achieved in 5 days and was >10 times higher than FTC-TP in blood, genital, and rectal compartments.
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Dose Response for Starting and Stopping HIV Preexposure Prophylaxis for Men Who Have Sex With Men
Sharon M Seifert,David V. Glidden,Amie L. Meditz,Jose R Castillo-Mancilla,Edward M. Gardner,Julie A. Predhomme,Caitlin Rower,Brandon Klein,Becky Jo Kerr,L. Anthony Guida,Jia-Hua Zheng,Lane R. Bushman,Peter L. Anderson +12 more
TL;DR: In this paper, the number of daily tenofovir disoproxil fumarate/emtricitabine (TDF/FTC) doses required to achieve and maintain (after discontinuation) intracellular drug concentrations that protect against human immunodeficiency virus (HIV) infection for men who have sex with men (MSM).
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Quantitation of Tenofovir and Emtricitabine in Dried Blood Spots (DBS) with LC-MS/MS
Jia-Hua Zheng,Louis A. Guida,Caitlin Rower,Jose R Castillo-Mancilla,Amie L. Meditz,Brandon Klein,Becky Jo Kerr,Jacob A. Langness,Lane R. Bushman,Jennifer J. Kiser,Peter L. Anderson +10 more
TL;DR: A reversed-phase high performance liquid chromatographic (LC), tandem mass spectrometry (MS/MS) assay for the determination of tenofovir and emtricitabine in dried blood spots (DBS) from human whole blood was developed and validated, indicating that DBS can be used as a plasma alternative for pharmacokinetic analyses in vivo.