Showing papers by "Brenda J. Tripathi published in 2004"
TL;DR: This paper investigated the mRNA and protein expression of fibronectin and stromelysin-1 (matrix metalloproteinase-3, MMP-3) by trabecular cells treated with growth factors present in primary and secondary aqueous humors.
16 citations
TL;DR: The hypothesis that the pathogenesis of PEX can be linked to disturbed metabolism of GAGs and PGs is supported and the blood-aqueous barrier was probably not significantly compromised in PEX patients with cataract but without open-angle glaucoma.
Abstract: Purpose. To investigate alterations in the proteoglycan (PG) and glycosaminoglycan (GAG) content of the aqueous humour in patients with pseudoexfoliation syndrome (PEX). Materials and methods. Aqueous humor samples were obtained during cataract surgery from nineteen patients bearing PEX features and twenty-three age-matched normal controls. Protein and IgG were quantified densitometrically after their electrophoretic separation. Collagen type IX, 3-sulphoglucuronic acid (HNK-1 epitope), biglycan and heparan sulphate proteoglycans were detected in Western and dot blots by using specific monoclonal antibodies (MAbs). The immunochemical analysis was performed in native aqueous humour or after degradation of the glycosaminoglycans with chondroitinases. Results. Degradation of the samples with chondroitinases ABC, AC and B revealed that, in the aqueous humour from PEX eyes, collagen type IX and biglycan had a more dermatan sulphate than did normal eyes. In addition, more HNK-1 epitope was observed in PEX eyes,...
13 citations
TL;DR: The ultrastructural features of the lens capsule in a patient with Kniest dysplasia are reported and it is reported that cataracts tend to develop at an early age and are firm in consistency.
Abstract: Kniest dysplasia is a variant of the spondyloepiphyseal dysplasias caused by an abnormal synthesis of collagen type II. In 1952, Kniest reported the first specific description of a variety of chondrodystrophy that he termed an atypical chondrodystrophy, which came to be known as Kniest dysplasia. A patient with Kniest dysplasia usually is seen with characteristic round facies, midfacial flatness, and proptosis. Radiologic findings are pathognomic and differentiate this syndrome fromotherbonedysplasias and dwarfism. Histologically the syndrome is characterized by abnormal cartilage with large chondrocytes embedded in loosely woven matrix that containsmanyemptyspacesandgives rise to the name Swiss cheese cartilage syndrome.Thechondrocyteshave dilated cisternae of endoplasmic reticulum. Abnormal organization of type II collagen has been found to be due to a gene mutation. Kniest dysplasia is associated with multiple ocular abnormalities. Myopia, vitreous liquefactionandsyneresis, vitreous condensation, traction at the vitreous base, areas of white without pressure at the retinal periphery, and extensive perivascular lattice degeneration are common findings. Cataracts tend to develop at an early age and are firm in consistency. We report the ultrastructural features of the lens capsule in a patient with Kniest dysplasia.
6 citations