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Brian Agnew
Researcher at Life Technologies
Publications - 6
Citations - 681
Brian Agnew is an academic researcher from Life Technologies. The author has contributed to research in topics: Actin depolymerizing factor & Glycoprotein. The author has an hindex of 4, co-authored 6 publications receiving 665 citations. Previous affiliations of Brian Agnew include Colorado State University.
Papers
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Journal ArticleDOI
Reactivation of phosphorylated actin depolymerizing factor and identification of the regulatory site.
TL;DR: Alkaline phosphatase treatment of one Asp-N peptide converted it to a peptide of mass 673, demonstrating that this peptide contains the phosphate group, confirming that Ser3 in the encoded ADF is the single in vivo regulatory site.
Journal ArticleDOI
Xenopus Actin Depolymerizing Factor/Cofilin (XAC) Is Responsible for the Turnover of Actin Filaments in Listeria monocytogenes Tails
TL;DR: Observations show that XAC but not gelsolin is essential for depolymerizing actin filaments that rapidly turn over in Xenopus extracts, and suggests that the substrate for XAC is the ADP-bound subunit of actin and that the lifetime of a filament is controlled by its nucleotide content.
Patent
Oligosaccharide modification and labeling of proteins
TL;DR: In this paper, the present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified OCCs.
Patent
Labeling and detection of post translationally modified proteins
TL;DR: In this article, new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules, or solid support were presented, and methods for enzymatically labeling a biomolecules with an azide group.
Patent
Methods for modifying and labeling protein with oligosaccharide
TL;DR: In this paper, a method for modifying proteins and antibodies by oligosaccharide conjugation was proposed, which comprises the steps of conjugating a chemical handle comprising a modified sugar to a GlcNAc residue on a first protein; and mixing the first protein with a reporter molecule, carrier molecule or solid support that is reactive with the chemical handle.