Reactivation of phosphorylated actin depolymerizing factor and identification of the regulatory site.
Reads0
Chats0
TLDR
Alkaline phosphatase treatment of one Asp-N peptide converted it to a peptide of mass 673, demonstrating that this peptide contains the phosphate group, confirming that Ser3 in the encoded ADF is the single in vivo regulatory site.About:
This article is published in Journal of Biological Chemistry.The article was published on 1995-07-21 and is currently open access. It has received 377 citations till now. The article focuses on the topics: Actin depolymerizing factor & Peptide sequence.read more
Citations
More filters
Journal ArticleDOI
ROCKs: multifunctional kinases in cell behaviour
TL;DR: Recent experiments have defined new functions of ROCKs in cells, including centrosome positioning and cell-size regulation, which might contribute to various physiological and pathological states.
Journal ArticleDOI
Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase.
Midori Maekawa,Toshimasa Ishizaki,Shuken Boku,Naoki Watanabe,Akiko Fujita,Akihiro Iwamatsu,Takashi Obinata,Kazumasa Ohashi,Kensaku Mizuno,Shuh Narumiya +9 more
TL;DR: Results indicate that phosphorylation of LIM-kinase by ROCK and consequently increased phosphorylated of cofilin by LIM- Kinase contribute to Rho-induced reorganization of the actin cytoskeleton.
Journal ArticleDOI
Molecular Mechanisms Controlling Actin Filament Dynamics in Nonmuscle Cells
TL;DR: How motile cells regulate actin filament assembly at their leading edge is reviewed, including how Arp2/3 complex is incorporated into the network, and new filaments are capped rapidly, so that activated Arp1/2 complex must be supplied continuously to keep the network growing.
Journal ArticleDOI
Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase
Silvia Arber,Freda A. Barbayannis,Hartwig Hanser,Corinna Schneider,Clement A. Stanyon,Ora Bernard,Pico Caroni +6 more
TL;DR: A mechanism for the regulation of cofilin and hence of actin dynamics in vivo is defined and should play a central role in regulating cell motility and morphogenesis by modulating the stability of act in cytoskeletal structures.
Journal ArticleDOI
Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization
Neng Yang,Osamu Higuchi,Kazumasa Ohashi,Kyoko Nagata,Atsushi Wada,Kenji Kangawa,Eisuke Nishida,Kensaku Mizuno,Kensaku Mizuno +8 more
TL;DR: It is shown that LIM-kinase 1 (LIMK-1), a serine/threonine kinase containing LIM and PDZ domains, phosphorylates cofilin at Ser’3, both in vitro and in vivo, which indicates that LIMK- 1 participates in Rac-mediated actin cytoskeletal reorganization, probably by phosphoryLating co Filin.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.